| 货号 | 2548S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | EDC4/Ge-1 Antibody detects endogenous levels of total EDC4/Ge-1 protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:400) |
| 供应商 | CST |
| 背景 | Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjögrens syndrome patient later diagnosed with primary biliary cirrhosis (1). EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation (2). Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182 (2,3). Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression (4). |
| 存放说明 | -20C |
| 计算分子量 | 170 |
Confocal immunofluorescent analysis of HeLa cells using EDC4/Ge-1 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). 使用EDC4/Ge-1 Antibody（绿色），共聚焦免疫荧光观察EDC4/Ge-1蛋白在HeLa细胞定位。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩 = DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from 293 and Hep G2 cells using EDC4/Ge-1 Antibody. 使用EDC4/Ge-1 Antibody，免疫印迹（Western Blotting）分析293和HepG2细胞系裂解物。 |
