| 货号 | 3503S |
| 反应种属 | Human/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Cortactin (H222) Antibody detects endogenous levels of total cortactin protein. |
| 使用方法 | WB(1:1000) IP (1:125) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4). Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8). |
| 存放说明 | -20C |
| 计算分子量 | 80-85 |
Confocal immunofluorescent analysis of HeLa cells using Cortactin (H222) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Cortactin (H222) Antibody (绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5™（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell types using Cortactin (H222) Antibody. 使用Cortactin (H222) Antibody，免疫印迹（Western Blot）分析不同细胞系中Cortactin蛋白水平。 | |
Immunoprecipitation of cortactin from HeLa cell lysates using Cortactin (H222) Antibody. Western blot was performed using the same antibody. 使用Cortactin (H222) Antibody,免疫沉淀HeLa细胞裂解物的cortactin蛋白。使用同样的抗体进行免疫印迹（Western blot）实验。 |
