| 货号 | 2225S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Caspase-1 Antibody detects endogenous levels of pro-caspase-1 and the caspase-1 p20 subunit. The antibody is expected to detect alpha, beta, gamma and delta isoforms. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Caspase-1, or interleukin-1ß converting enzyme (ICE/ICEα), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12. Caspase-1 cleaves inflammatory cytokines such as pro-IL-1ß and interferon-γ inducing factor (IL-18) into their mature forms (1,2). Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10. Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD). Overexpression of caspase-1 can induce apoptosis (3). Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1β and are resistant to endotoxic shock (4,5). At least six caspase-1 isoforms have been identified, including caspase-1 α, β, γ, δ, ε and ζ (6). Most caspase-1 isoforms (α, β, γ and δ) produce products between 30-48 kDa and induce apoptosis upon over-expression. Caspase-1 ε typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative. The widely expressed ζ isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the α isoform (6). Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7). |
| 存放说明 | -20C |
| 计算分子量 | 20 p20. 30 to 45 beta, delta, gamma. 50 alpha. |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Caspase-1 Antibody. 免疫组织化学分析石蜡包埋的人肺癌切片，使用抗体为Caspase-1 Antibody。 | |
Western blot analysis of extracts from THP-1 and HL-60 cells, using Caspase-1 Antibody. Western blot 检测THP-1和HL-60 细胞系提取物，使用抗体为Caspase-1 Antibody。 |
