| 货号 | 4128S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IF-F/IF-IC |
| 使用方法 | WB(1:1000) IF-F (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a multifunctional protein with several biological activities. These include roles in DNA repair and in the cellular response to oxidative stress. Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway (1). Repair activities of Ape1 are stimulated by interaction with XRCC1 (2), another essential protein in BER. Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation (3). Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors (4). Ape1 has been shown to stimulate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-κB, PAX, HIF-1, HLF and p53 (4). Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS) (5,6).Ape1(脱嘌呤/脱嘧啶核酸内切酶1)，又称Ref1(氧化还原效应因子1)，是一种多功能蛋白，具有多种生物学活性，主要包括在DNA修复和细胞氧化应激反应中的作用。Ape1启动脱碱基位点的修复并在碱基切除修复(BER)路径中发挥重要作用(1)。Ape1的修复活性通过与BER中另外一个关键蛋白XRCC1 (2)相互作用被激活。作为一个重要的氧化还原因子，Ape1不仅可以维持转录因子在一个活跃还原的状态，也可以独立于氧化还原的形式，作为转录辅助因子控制细胞命运，如细胞凋亡、增殖和分化(3)。Ape1表达的升高和多种类型的肿瘤相关，如子宫颈癌、卵巢癌、前列腺癌、横纹肌肉瘤和生殖细胞肿瘤(4)。Ape1可以促进DNA结合肿瘤发生过程中几个重要的转录因子如Fos, Jun, NF-κB, PAX, HIF-1, HLF 和 p53 (4)。Ape1基因的突变也和肌萎缩侧索硬化症有关(5,6)。 |
| 存放说明 | -20C |
| 计算分子量 | 34 |
Confocal immunofluorescent analysis of normal mouse cerebellum (left) and hippocampus (right) using Ape1 Antibody (green) and Neurofilament-L (DA2) Mouse mAb #2835 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测正常小鼠脑组织， 左图为小脑组织，右图为海马组织，检测抗体为Ape1 抗体(绿色)和神经纤维丝抗体Neurofilament-L (DA2) Mouse mAb #2835(红色)。蓝色伪彩为DNA荧光染料（产品信息为 DRAQ5®#4084 ）。 | |
Western blot analysis of extracts from various cell types using Ape1 Antibody.Western blot方法检测不同细胞(从左向右依次为Hela，Jurkat，PC-3，H1975，HCC827)提取物中Ape1蛋白水平，使用的抗体为Ape1 抗体。 |
