| 货号 | 4642S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Apoptosis-inducing factor (AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (reviewed in 1,2). AIF is normally localized to the mitochondrial intermembrane space and released in response to apoptotic stimuli (3). Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events such as chromatin condensation and large-scale DNA fragmentation (3). Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis (4). Structural analysis of AIF revealed two important regions, the first having oxidoreductase activity and the second being a potential DNA binding domain (3,5). While AIF is redox-active and can behave as an NADH oxidase, this activity is not required for inducing apoptosis (6). Instead, recent studies suggest that AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity (2,7). |
| 存放说明 | -20C |
| 计算分子量 | 57, 67 |
Western blot analysis of extracts from human (Jurkat), mouse (NIH/3T3), and rat (NBT-II) cell lines, using AIF Antibody. Western blot分析人(Jurkat)、小鼠(NIH/3T3)和大鼠(NBT-II)细胞系提取物，使用抗体为AIF Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, showing perinuclear and cytoplasmic localization, using AIF Antibody. 免疫组化分析石蜡包埋的人前列腺癌切片，显示核周和细胞质的定位，使用AIF Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AIF Antibody. 免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为AIF Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using AIF Antibody. 免疫组织化学分析石蜡包埋的人乳腺癌切片，使用抗体为AIF Antibody。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using AIF Antibody. 免疫组织化学分析石蜡包埋的人肺癌切片，使用抗体为AIF Antibody。 | |
Confocal immunofluorescent images of HeLa cells labeled with AIF Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa细胞，使用抗体为AIF Antibody （绿色），MitoTracker® Red CMXRos 标记线粒体（红色），与其共定位。蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
