| 货号 | 94808S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | MMP13 Antibody recognizes endogenous levels of total MMP13 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | MMP13 (collagenase 3) belongs to the matrix metalloproteinase (MMP) superfamily of enzymes that targets many extracellular proteins, including other proteases, growth factors, cell surface receptors, and adhesion molecules (1, 2). MMP13 is a member of a subgroup of collagenases (including MMP1, MMP8, and MMP18) that play an even more important function targeting fibrillar collagen. MMP13 is synthesized as a latent proenzyme, and proteolytic removal of the inhibitory propeptide domain is required for enzyme activation. MMP13 protein levels are regulated at the transcriptional level, via specific transcription factors and via promoter DNA methylation (3, 4). MMP13 preferentially cleaves Type II collagen, and research studies have shown that aberrant upregulation of MMP13 activity can lead to cartilage loss and osteoarthritis (5, 6). In addition, MMP13 has been shown to promote cancer development, in part through enhancing tumor angiogenesis and metastases (7-9), suggesting that collagenase activity may serve as a useful marker of tumor progression (10). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
| 参考文献 | 1 . McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40. 2 . Cathcart, J. et al. (2015) Genes Dis 2, 26-34. 3 . Chan, C.M. et al. (2017) J Biol Chem 292, 1625-1636. 4 . Hashimoto, K. et al. (2013) J Biol Chem 288, 10061-72. 5 . Elayyan, J. et al. (2017) FASEB J , . 6 . Wang, M. et al. (2011) Ann N Y Acad Sci 1240, 61-9. 7 . Mendonsa, A.M. et al. (2015) Mol Cancer 14, 49. 8 . Wang, C. et al. (2015) Oncotarget 6, 2903-16. 9 . Kudo, Y. et al. (2012) J Biol Chem 287, 38716-28. 10 . Brinckerhoff, C.E. et al. (2000) Clin Cancer Res 6, 4823-30. |
Western blot analysis of extracts from concentrated culture medium of LN18 and ScaBER cell lines using MMP13 Antibody. |
