| 货号 | 9527T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C only when phosphorylated at Thr48. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活，该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化，而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时，cdc25C结合14-3-3蛋白家族的成员，扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 75 |
| 参考文献 | 1 . Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228. 2 . Peng, C.Y. et al. (1997) Science 277, 1501-1505. 3 . Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072. 4 . Blasina, A. et al. (1999) Curr. Biol. 9, 1-10. 5 . Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845. 6 . Izumi, T. and Maller, J.L. (1993) Mol Biol Cell 4, 1337-50. 7 . Stukenberg, P.T. and Kirschner, M.W. (2001) Mol Cell 7, 1071-83. 8 . Yaffe, M.B. et al. (1997) Science 278, 1957-60. 9 . Lu, P.J. et al. (1999) Science 283, 1325-8. 10 . Landrieu, I. et al. (2001) J Biol Chem 276, 1434-8. |
Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated and lambda phosphotase-treated using Phospho-cdc25C (Thr48) (upper), and cdc25C (5H9) Rabbit mAb, #4688 (lower).western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT29细胞提取物，使用的抗体为Phospho-cdc25C (Thr48) (上图),和cdc25C (5H9) Rabbit mAb, #4688 (下图) |
