| 货号 | 9551T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-PTEN (Ser380) Antibody detects endogenous levels of PTEN only when phosphorylated at Ser380 |
| 使用方法 | WB(1:1000) IP (1:25) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10) |
| 存放说明 | -20C |
| 计算分子量 | 54 |
| 参考文献 | 1 . Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 2 . Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. 3 . Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 4 . Wan X and Helman LJ (2003) Oncogene 22, 8205–11 5 . Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. 6 . Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. 7 . Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. 8 . Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. 9 . Funamoto, S. et al. (2002) Cell 109, 611-23. 10 . Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610. |
Western blot analysis of extracts from various cell lines, using Phospho-PTEN (Ser380) Antibody (upper) or PTEN Antibody #9552 (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer. Western blot 分析不同细胞系的提取物，所用抗体为Phospho-PTEN (Ser380) Antibody (上) 或 PTEN Antibody #9552 (下)。抗体的磷酸特异性是通过western 转膜后，采用牛肠磷酸酶(CIP)处理膜证实的。 |
