| 货号 | 9491T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Cleaved Caspase-7 (Asp198) Antibody detects endogenous levels of the large fragment of caspase-7 resulting from cleavage at aspartic acid 198. The antibody does not cross-react with full length caspase-7 or with other caspases. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4). |
| 存放说明 | -20C |
| 计算分子量 | 20 |
| 参考文献 | 1 . Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52. 2 . Duan, H. et al. (1996) J Biol Chem 271, 1621-5. 3 . Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8. 4 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 5 . Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11. |
Western blot analysis of extracts from Jurkat cells and 293 cells, untreated, cytochrome c-treated (0.25 mg/ml, 1 hour) or etoposide-treated (25 µM, 5 hours), using Cleaved Caspase-7 (Asp198) Antibody. 采用Cleaved Caspase-7 (Asp198) Antibody对未处理或 cytochrome c（细胞色素c）处理(0.25 mg/ml, 1 hour) 或 etoposide （依托泊苷）处理（25 µM, 5 hours）的 Jurkat 和 293 细胞进行 western blot 分析。 |
