| 货号 | 2441T |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Phospho-eIF4G (Ser1108) Antibody detects eIF4GI only when phosphorylated at Ser1108. It does not cross-react with nonphosphorylated eIF4GI or p97. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:600) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. |
| 存放说明 | -20C |
| 计算分子量 | 220 |
| 参考文献 | 1 . Sonenberg, N. et al. (1978) Proc. Natl. Acad. Sci. USA 75, 4843-4847. 2 . Gingras, A.C. et al. (1999) Annu. Rev. Biochem. 68, 913-963. 3 . Waskiewicz, A. et al. (1999) Mol. Cell. Biol. 19, 1871-1880. 4 . Pyronnet, S. et al. (1999) EMBO J. 18, 270-279. 5 . Raught, B. et al. (2000) EMBO J. 19, 434-444. |
Western blot analysis of extracts from PC12 cells, untreated (lane 1), NGF-treated (10 ng/ml) (lane 2), anisomycin-treated (25 µM) (lane 3), U0126-treated #9903 (10 µM) (lane 4), Rapamycin-treated #9904 (100 nM ) (lane 5) or LY294002-treated #9901 (25 µM) (lane 6), using Phospho-eIF4G (Ser1108) Antibody. 使用Phospho-eIF4G (Ser1108) Antibody，免疫印迹（Western Blot）分析在PC12细胞系中Phospho-eIF4G (Ser1108)蛋白水平，分别分为对照组（第1道），NGF处理组(10 ng/ml) （第2道），anisomycin处理组(25 µM) （第3道），U0126处理组 #9903 (10 µM) （第4道），Rapamycin处理组#9904 (100 nM ) (第5道)和LY294002处理组 #9901 (25 µM) (第6道)。 | |
Western blot analysis of extracts from 293 cells expressing GST-eIF4GI Ser1192Ala or GST-eIF4GI Ser1108Ala mutant protein, using Phospho-eIF4G (Ser1108) Antibody. (Provided by Brian Raught, McGill University, Montreal, QuŽbec.) 使用Phospho-eIF4G (Ser1108) Antibody，免疫印迹（Western Blot）分析在293细胞系中GST-eIF4GI Ser1192Ala和GST-eIF4GI Ser1108Ala突变体蛋白的水平。(Provided by Brian Raught, McGill University, Montreal, QuŽbec.) | |
Confocal immunofluorescent analysis of HeLa cells either rapamycin-treated (left) or serum-treated (right), using Phospho-eIF4G (Ser1108) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 使用Phospho-eIF4G (Ser1108) Antibody（绿色），共聚焦免疫荧光观察Phospho-eIF4G (Ser1108)蛋白在HeLa细胞中定位，细胞分为rapamycin处理组（左图）和血清处理组（右图）。DY-554 phalloidin (红色)标记微丝蛋白。蓝色伪彩= DRAQ5™ (DNA荧光染料) |
