| 货号 | 2381T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-IRS-1 (Ser307) Antibody detects endogenous IRS-1 only when phosphorylated at serine 307. This antibody does not cross-react with other related phospho-proteins. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). |
| 存放说明 | -20C |
| 计算分子量 | 180 |
| 参考文献 | 1 . Sun, X.J. et al. (1991) Nature 352, 73-77. 2 . Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672. 3 . Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430. 4 . Wang, L.M. et al. (1993) Science 261, 1591-1594. 5 . Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189. 6 . Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121. 7 . Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447. 8 . Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645. 9 . De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947. 10 . Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307. |
Immunoprecipitation of IRS-1 from differentiated C2C12 cell extracts, untreated or insulin-treated for the indicated times, using IRS-1 Antibody #2382, followed by Western blot with either Phospho-IRS-1 (Ser307) Antibody, or IRS-1 Antibody #2382. 对分化的C2C12细胞抽提液，未处理或胰岛素处理适当时间，使IRS-1 Antibody #2382进行免疫共沉淀分析，然后用Phospho-IRS-1 (Ser307) Antibody或IRS-1 Antibody #2382进行Western blot分析。 | |
Western blot analysis of extracts from CHO IR/IRS-1 cells (transfected with insulin receptor and IRS-1), untreated or insulin-treated (100 nM for 5 min), showing an increase in phospho-IRS-1 (Ser307) with insulin stimulation, using Phospho-IRS-1 (Ser307) Antibody. 对CHO IR/IRS-1细胞（转染胰岛素受体和IRS-1）抽提液，未处理或100nM胰岛素处理5分钟，使用Phospho-IRS-1 (Ser307) Antibody进行Western blot分析，显示胰岛素刺激后phospho-IRS-1 (Ser307)水平上升。 | |
Western blot analysis of MCF-7 cell extracts, unstimulated and insulin-stimulated (100 nM for 5 min), using IRS-1 Antibody #2382 (left) and Phospho-IRS-1 (Ser307) Antibody (right). 对MCF-7细胞（未处理或100nM胰岛素处理5分钟），使用IRS-1 Antibody #2382（左）或Phospho-IRS-1 (Ser307) Antibody（右）进行Western blot分析。 |
