| 货号 | 4241T |
| 反应种属 | Human,Mouse,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | CHD3 Antibody detects endogenous levels of total CHD3 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multi-protein complexes, with their transcriptional activation or repression activity governed by other proteins also found in the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RbAp48, RbAp46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two PHD (plant homeodomain) zinc finger domains that bind directly to HDAC1 and HDAC2. |
| 存放说明 | -20C |
| 计算分子量 | 260 |
| 参考文献 | 1 . Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76. 2 . Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40. 3 . Tong, J.K. et al. (1998) Nature 395, 917-21. 4 . Xue, Y. et al. (1998) Mol Cell 2, 851-61. 5 . Zhang, Y. et al. (1998) Cell 95, 279-89. 6 . Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7. 7 . Jones, P.L. et al. (1998) Nat Genet 19, 187-91. 8 . Fujita, N. et al. (2003) Cell 113, 207-19. |
Western blot analysis of extracts from HeLa and Jurkat cells using CHD3 Antibody. 使用CHD3 Antibody，免疫印迹(Western blot)分析HeLa和Jurkat细胞中CHD3的蛋白水平。 |
