货号 | MAB22772-SP |
别名 | 3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 | 全称 | Interleukin 8 |
反应种属 | Feline |
应用 | ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.5-2.0 µg/mL ) ELISA Standard ( ) |
目标/特异性 | Detects feline CXCL8/IL‑8 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human CXCL8, recombinant canine CXCL8, or recombinant porcine CXCL8 is observed. |
使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.075-0.375 µg/mL in the presence of 20 ng/mL Recombinant Feline CXCL8/IL‑8. |
来源 | Monoclonal Rat IgG2A Clone # 344615 |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant feline CXCL8/IL‑8 Ala23-Ala101 Accession # Q9XSX5.1 |
生物活性 | Feline |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | CXCL8/Interleukin-8 is a chemokine that is upregulated at sites of inflammation where it promotes neutrophil infiltration and activation. CXCL8 can form homodimers and heterodimers with CXCL4/PF4. Its bioactivity is regulated by proteolytic truncations, citrullination, and the decoy receptor DARC. CXCL8 signals through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin which prevents CXCL8 interaction with CXCR1. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
|
Chemotaxis Induced by CXCL8/IL‑8 and Neutralization by Feline CXCL8/IL‑8 Antibody. Recombinant Feline CXCL8/IL‑8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline CXCL8/IL‑8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Feline CXCL8/IL‑8 Monoclonal Antibody (Catalog # MAB22772). The ND50 is typically 0.075‑0.375 µg/mL. |