| 货号 | MAB2177-SP |
| 别名 | Activation-inducible TNF-related ligand; AITRLMGC138237; GITRLglucocorticoid-induced TNFR-related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRLGITR ligand; TL6AITR ligand; TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18 | 全称 | Glucocorticoid Induced TNF Receptor Family Related Gene Ligand |
| 反应种属 | Mouse |
| 应用 | ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human GITR Ligand, recombinant mouse (rm) CD40, or rmTNF-alpha is observed. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize GITR Ligand/TNFSF18-induced proliferation in mouse CD4+ T cells. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 2 µg/mL Recombinant Mouse GITR Ligand/TNFSF18, 0.5 µg/mL Mouse CD3 epsilon Monoclonal Antibody, and 10 µg/mL of a cross-linking antibody, polyHistidine Monoclonal Antibody. |
| 来源 | Monoclonal Rat IgG2A Clone # 337122 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8995 (Human); 240873 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse GITR Ligand/TNFSF18 Thr47-Ser173 Accession # Q80YG2 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) residues type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently up-regulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25+ regulatory CD4+ T cells (Treg). GITR is also expressed on naïve CD4+CD25-T cells, where its expression is up-regulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides co-stimulatory signals for activated CD4+CD25-T cells to enhance cell proliferation and augment cytokine production. On CD4+CD25+ Treg cells, GITRL also provides co-stimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important co-stimulatory signals that play important roles in modulating diverse T cell functions (1‑4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Proliferation Induced by GITR Ligand/TNFSF18 and Neutralization by Mouse GITR Ligand/TNFSF18 Antibody. In the presence of Mouse CD3 epsilon Monoclonal Antibody (0.5 µg/mL, Catalog # MAB484) and a cross-linking antibody, polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Mouse GITR Ligand/TNFSF18 (Catalog # 2177-GL) stimulates proliferation in mouse CD4+ T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse GITR Ligand/TNFSF18 (2 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GITR Ligand/ TNFSF18 Monoclonal Antibody (Catalog # MAB2177). The ND50 is typically 2-8 µg/mL. |
