| 货号 | MAB2656-SP |
| 别名 | ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin | 全称 | Sex Hormone-binding Globulin |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human SHBG in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6462 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human SHBG Leu30-His402 Accession # P04278 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Human SHBG by Western Blot. Western blot shows lysates of human testis tissue, human liver tissue, and human plasma. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB2656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SHBG at approximately 50-53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
