Human IGFBP-1 MAb (Clone 33627) (25 UG)【科瑞奥博】

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Human IGFBP-1 MAb (Clone 33627) (25 UG)

货号： MAB675-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB675-SP
别名	alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP
全称	Insulin-like Growth Factor Binding Protein 1
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human IGFBP-1 in ELISAs and Western blots. In Western blots, this antibody does not cross‑react with recombinant human (rh) IGFBP‑2, rhIGFBP‑3, or rhIGFBP‑4.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IGFBP‑1 inhibition of IGF‑I-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND₅₀) is typically 10‑40 µg/mL in the presence of 5 µg/mL Recombinant Human IGFBP‑1 and 6 ng/mL Recombinant Human IGF‑I.
来源	Monoclonal Mouse IgG₁ Clone # 33627
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3484 (Human); 16006 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Glioblastoma-derived Macrophage Colony-stimulating Factor (MCSF) Induces Microglial Release of Insulin-like Growth Factor-binding Protein 1 (IGFBP1) to Promote Angiogenesis. Authors: Nijaguna M, Patil V, Urbach S, Shwetha S, Sravani K, Hegde A, Chandramouli B, Arivazhagan A, Marin P, Santosh V, Somasundaram K J Biol Chem, 2015;290(38):23401-15. Species: Human Sample Type: Whole Cells Application: Neut Evaluation of human first trimester decidual and telomerase-transformed endometrial stromal cells as model systems of in vitro decidualization. Authors: Saleh L, Otti GR, Fiala C, Pollheimer J, Knofler M Reprod. Biol. Endocrinol., 2011;9(0):155. Species: Human Sample Type: Cell Lysates Application: WB Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ Transplantation, 2009;87(12):1814-20. Species: Human Sample Type: Urine Application: Antibody Array Development Biological variation in fasting serum insulin-like growth factor binding protein-1 (IGFBP-1) among individuals with a varying glucose tolerance. Authors: Borai A, Livingstone C, Mehta S, Zarif H, Abdelaal F, Ferns G Clin. Biochem., 2009;0(0):. Species: Human Sample Type: Serum Application: ELISA Development Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A Biochem. Biophys. Res. Commun., 2005;333(3):1011-6. Species: Human Sample Type: recombinant IGFBP-1 Application: WB
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IGFBP-1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-1 cDNA encodes a 259 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 234 aa residue mature protein. IGFBP-1 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. IGFBP-1 is expressed in liver, decidua, kidneys and is the most abundant IGFBP in amniotic fluid. Serum levels of IGFBP-1 are lowest after meals. Hepatocyte production of IGFBP-1 is regulated at the transcriptional level due to the affects of insulin and corticosteriods. IGFBP-1 binds equally well to IGF-I and IGF-II, with phosphorylated forms of IGFBP-1 exhibiting higher binding affinities.
运输条件	Blue Ice
存放说明	4℃
参考文献	Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3. Kelley, K.M.et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

参考图片

IGFBP‑1 Inhibition of IGF‑I-dependent Cell Proliferation and Neutralization by Human IGFBP‑1 Antibody. Recombinant Human IGFBP‑1 (Catalog # 871-B1) inhibits Recombinant Human IGF‑I (Catalog # 291-G1) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑I (6 ng/mL) activity elicited by Recombinant Human IGFBP‑1 (5 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675). The ND₅₀ is typically 10-40 µg/mL.