| 货号 | MAB193-SP |
| 别名 | B cell maturation antigen; B-cell maturation protein; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17 | 全称 | B Cell Maturation Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human BCMA in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross‑reactivity with recombinant mouse (rm) BCMA or rmTACI. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.2 - 0.8 μg/mL of this antibody will block 50% of the binding of 500 ng/mL of recombinant human APRIL to immobilized recombinant human BCMA coated at 1 μg/mL (100 μL/well). At 5 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG2A Clone # 335004 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 608 (Human); 21935 (Mouse); 287034 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human BCMA Met1-Ala54 Accession # Q02223 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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