Human IGF-I R MAb (Clone 33255) (25 UG)【科瑞奥博】

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Human IGF-I R MAb (Clone 33255) (25 UG)

货号： MAB391-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB391-SP
别名	CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-I receptor; IGFIR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2
全称	Insulin-like Growth Factor I Receptor
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human IGF-I R in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta RII.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IGF‑I-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. At 11 µg/mL, this antibody will neutralize approximately 50-75% of the bioactivity due to 6 ng/mL Recombinant Human IGF‑I.
来源	Monoclonal Mouse IgG₁ Clone # 33255
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3480 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes Authors: M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter Elife, 2016;5(0):. Species: Human Sample Type: Cell Lysates Application: WB Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Authors: Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S Mol Brain, 2015;8(0):51. Species: Human Sample Type: Whole Cells Application: Neut Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity. Authors: Jacques C, Holzenberger M, Mladenovic Z J. Biol. Chem., 2012;287(18):15100-8. Species: Human Sample Type: Whole Cells Application: Neut Expression of the IGF axis is decreased in local prostate cancer but enhanced after benign prostate epithelial differentiation and TGF-beta treatment. Authors: Massoner P, Ladurner Rennau M, Heidegger I, Kloss-Brandstatter A, Summerer M, Reichhart E, Schafer G, Klocker H Am. J. Pathol., 2011;179(6):2905-19. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Autologous bone marrow stromal cells genetically engineered to secrete an igf-I receptor decoy prevent the growth of liver metastases. Authors: Wang N, Fallavollita L, Nguyen L, Burnier J, Rafei M, Galipeau J, Yakar S, Brodt P Mol. Ther., 2009;17(7):1241-9. Species: Human Sample Type: Plasma Application: ELISA Development Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes. Authors: Back K, Arnqvist HJ Growth Horm. IGF Res., 2009;19(2):101-11. Species: Human Sample Type: Cell Lysates Application: ELISA Development Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels. Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U Leuk. Res., 2008;32(8):1244-58. Species: Human Sample Type: Whole Cells Application: Flow Insulin-like growth factor (IGF) 2 stimulates steroidogenesis and mitosis of bovine granulosa cells through the IGF1 receptor: role of follicle-stimulating hormone and IGF2 receptor. Authors: Spicer LJ, Aad PY Biol. Reprod., 2007;77(1):18-27. Species: Bovine Sample Type: Whole Cells Application: WB Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts. Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U Stem Cells, 2007;25(8):1862-71. Species: Human Sample Type: Whole Cells Application: Flow Mesenchymal stem cell abnormalities in patients with multiple myeloma. Authors: Garderet L, Mazurier C, Chapel A, Ernou I, Boutin L, Holy X, Gorin NC, Lopez M, Doucet C, Lataillade JJ Leuk. Lymphoma, 2007;48(10):2032-41. Species: Human Sample Type: Whole Cells Application: Flow The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle. Authors: Quirk SM, Cowan RG, Harman RM J. Endocrinol., 2006;189(3):441-53. Species: Bovine Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R Glu31-Asn932 Accession # P08069
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cancer Biomarkers Glucose Homeostasis Human CD Marker Products ICC / IHC Images: IGF-I R IGF Family Insulin-like Growth Factor (IGF) and Receptors Insulin-Like Growth Factors and Cancer Protein/Peptide Hormones and Regulators Receptor Tyrosine Kinases (RTKs) Receptor Tyrosine Kinases (RTKs) in the Akt Pathway Receptors in the Jak/STAT Pathway

参考图片

Cell Proliferation Induced by IGF‑I and Neutralization by Human IGF‑I R Antibody.

Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGF-I R Monoclonal Antibody (Catalog # MAB391). At 11 µg/mL, this antibody will neutralize 50-75% rhIGF-1 induced activity.

Detection of Human IGF‑I R by Western Blot.

Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I R Monoclonal Antibody (Catalog # MAB391) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IGF-I R at approximately 275 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.