Human CXCL10/IP-10 MAb (Clone 33036) (25 UG)【科瑞奥博】

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Human CXCL10/IP-10 MAb (Clone 33036) (25 UG)

货号： MAB266-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB266-SP
别名	C7; chemokine (C-X-C motif) ligand 10; CRG-2; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
反应种属	Human
应用	Western Blot,CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, does not cross-react with recombinant human CXCL1,2, 3, 5, 8, 9, 12/SDF-1 beta, recombinant mouse CXCL1, or 2.
使用方法	Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND₅₀) is typically 0.5-2.0 µg/mL in the presence of 0.2 µg/mL Recombinant Human CXCL10/IP‑10/CRG‑2.
来源	Monoclonal Mouse IgG₁ Clone # 33036
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3627 (Human); 15945 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016;6(0):29909. Species: Human Sample Type: Serum Escherichia coli Pyruvate Dehydrogenase Complex Is an Important Component of CXCL10-Mediated Antimicrobial Activity. Authors: Schutte K, Fisher D, Burdick M, Mehrad B, Mathers A, Mann B, Nakamoto R, Hughes M Infect Immun, 2015;84(1):320-8. Species: Human Sample Type: recombinant protein Application: immunoassay Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients. Authors: Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R Clin Cancer Res, 2013;19(7):1858-72. Species: Human Sample Type: Whole Cells Application: Functional Assay Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. Species: Human Sample Type: Whole Cells Application: Neut Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T Mod Pathol, 2012;26(3):357-69. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus. Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J J. Invest. Dermatol., 2011;131(1):133-40. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH J. Immunol., 2010;184(6):2886-98. Species: Human Sample Type: Whole Cells Application: Neut Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Authors: Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Authors: Franciszkiewicz K, Le Floch A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Cancer Res., 2009;69(15):6249-55. Species: Human Sample Type: Whole Cells Application: ICC Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. Authors: Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D J. Immunol., 2007;179(6):4272-82. Species: Human Sample Type: Whole Cells Application: Immunodepletion Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. Authors: Pfleger C, Schloot N, ter Veld F J. Immunol. Methods, 2007;329(1):214-8. Species: Human Sample Type: Serum Application: Luminex Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA J. Immunol., 2007;179(7):4840-8. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Eotaxin-2 and colorectal cancer: a potential target for immune therapy. Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE Clin. Cancer Res., 2007;13(19):5719-28. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Clin. Immunol., 2006;120(2):220-31. Species: Human Sample Type: blister fluid Application: Neut Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells. Authors: Edwards MR, Johnson MW, Johnston SL Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Simian human immunodeficiency virus-associated pneumonia correlates with increased expression of MCP-1, CXCL10, and viral RNA in the lungs of rhesus macaques. Authors: Sui Y, Li S, Pinson D, Adany I, Li Z, Villinger F, Narayan O, Buch S Am. J. Pathol., 2005;166(2):355-65. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Neut Enhanced type I interferon signalling promotes Th1-biased inflammation in cutaneous lupus erythematosus. Authors: Wenzel J, Worenkamper E, Freutel S, Henze S, Haller O, Bieber T, Tuting T J. Pathol., 2005;205(4):435-42. Species: Human Sample Type: Whole Cells Application: ICC Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D J. Immunol., 2004;173(11):6592-602. Species: Human Sample Type: Whole Tissue Application: IHC Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Blood, 2004;104(2):314-20. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees. Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T J. Infect. Dis., 1999;179(3):646-52. Species: Primate - Pan troglodytes (Chimpanzee) Sample Type: Plasma Application: ELISA Development
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CXCL10/IP-10/CRG-2 Val22-Pro98 Accession # P02778.2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.
运输条件	Blue Ice
存放说明	4℃
参考文献	Loetscher, M. et al. (1996) J. Exp. Med. 184:963. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.

参考图片

Chemotaxis Induced by CXCL10/IP‑10 and Neutralization by Human CXCL10/IP‑10 Antibody. Recombinant Human CXCL10/
IP‑10 (Catalog # 266‑IP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/
IP‑10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL10/IP‑10 Monoclonal Antibody (Catalog # MAB266). The ND₅₀ is typically 0.5-2.0 µg/mL.