| 货号 | MAB35661-SP |
| 别名 | AI2A1; ARM1; EC 3.4.21; EC 3.4.21.-; EMSP; EMSP1; EMSP1MGC116827; Enamel matrix serine proteinase 1; kallikrein 4 (prostase, enamel matrix, prostate); kallikrein-4; Kallikrein-like protein 1; kallikrein-related peptidase 4; KLK4; KLK-L1MGC116828; Prostase; PRSS17enamel matrix serine protease 1; PSTSandrogen-regulated message 1; Serine protease 17 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Kallikrein 4/Prostase/EMSP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Kallikrein 1, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhFactor VII, rhFactor X, rhFactor XI, rhHGFA, rhThrombin, and rhUPA-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Kallikrein 4/Prostase/EMSP1 (1.0 µg/mL, Catalog # 1719‑SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 2.9 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 325712 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9622 (Human) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Kallikrein 4/Prostase/EMSP1 Ser27-Ser254 Accession # NP_004908 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Kallikrein 4 (KLK4), also known as prostase or EMSP1 (enamel matrix serine protease 1), is a serine protease of the human tissue kallikrein gene family (1). Among normal tissues, human KLK4 is specifically expressed in the prostate (2). It is over-expressed in prostate cancer and this expression is regulated by hormones including androgens, esterogen and progesterone (3). Recombinant human KLK4 readily activates pro-KLK3/PSA and pro-urokinase type plasminogen activator (uPA), indicating it may initiate events involving PSA and uPA in either normal or abnormal processes (4). KLK4 may have additional roles such as functioning as one of the two major enamel proteases identified that process enamel matrix proteins (5). In addition to being a secreted enzyme, it is also a nuclear protein (3, 6). The deduced amino acid sequence of human KLK4 consists of a signal peptide, a short pro region and a mature/active enzyme. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Kallikrein 4/Prostase/EMSP1 in Human Prostate. Kallikrein 4/Prostase/EMSP1 was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human Kallikrein 4/Prostase/EMSP1 Monoclonal Antibody (Catalog # MAB35661) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
