Mouse IL-1 beta/IL-1F2 MAb (Clone 30311) (25 UG)【科瑞奥博】

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Mouse IL-1 beta/IL-1F2 MAb (Clone 30311) (25 UG)

货号： MAB401-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB401-SP
别名	catabolin; IL-1 beta; IL-1; IL1B; IL-1B; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
全称	Interleukin 1 beta
反应种属	Mouse
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects mouse IL-1 beta /IL-1F2 in ELISAs.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 50 pg/mL Recombinant Mouse IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
来源	Monoclonal Rat IgG₁ Clone # 30311
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. AMCase is a crucial regulator of type 2 immune responses to inhaled house dust mites. Authors: Kim L, Morita R, Kobayashi Y, Eisenbarth S, Lee C, Elias J, Eynon E, Flavell R Proc Natl Acad Sci U S A, 2015;112(22):E2891-9. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Capture Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. Authors: Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P J Immunol, 2015;194(1):334-48. Species: Mouse Sample Type: Serum Application: ELISA Development A tissue-specific role for Nlrp3 in tubular epithelial repair after renal ischemia/reperfusion. Authors: Bakker P, Butter L, Claessen N, Teske G, Sutterwala F, Florquin S, Leemans J Am J Pathol, 2014;184(7):2013-22. Species: Mouse Sample Type: Tissue Homogenates Application: IP Multiple CD11c+ cells collaboratively express IL-1beta to modulate stromal vascular endothelial growth factor and lymph node vascular-stromal growth. Authors: Benahmed F, Chyou S, Dasoveanu D, Chen J, Kumar V, Iwakura Y, Lu T J Immunol, 2014;192(9):4153-63. Species: Mouse Sample Type: In Vivo Application: In vivo NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Authors: Eisenbarth, Stephani, Williams, Adam, Colegio, Oscar R, Meng, Hailong, Strowig, Till, Rongvaux, Anthony, Henao-Mejia, Jorge, Thaiss, Christop, Joly, Sophie, Gonzalez, David G, Xu, Lan, Zenewicz, Lauren A, Haberman, Ann M, Elinav, Eran, Kleinstein, Steven H, Sutterwala, Fayyaz S, Flavell, Richard Nature, 2012;484(7395):510-3. Species: Mouse Sample Type: Cell Culture Supernates Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development The CDK domain of p21 is a suppressor of IL-1beta-mediated inflammation in activated macrophages. Authors: Scatizzi JC, Mavers M, Hutcheson J, Young B, Shi B, Pope RM, Ruderman EM, Samways DS, Corbett JA, Egan TM, Perlman H Eur. J. Immunol., 2009;39(3):820-5. Species: Mouse Sample Type: Cell Lysates Application: WB Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP J. Immunol., 2009;183(9):5799-806. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Stimulation of Toll-like receptor 3 and 4 induces interleukin-1beta maturation by caspase-8. Authors: Maelfait J, Vercammen E, Janssens S, Schotte P, Haegman M, Magez S, Beyaert R J. Exp. Med., 2008;205(9):1967-73. Species: Mouse Sample Type: Cell Lysates Application: WB TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor. Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ Eur. J. Pharmacol., 2007;573(1):221-9. Species: Mouse Sample Type: Tissue Homogenates Application: ELISA Development Maternal immune activation alters fetal brain development through interleukin-6. Authors: Smith SE, Li J, Garbett K, Mirnics K, Patterson PH J. Neurosci., 2007;27(40):10695-702. Species: Mouse Sample Type: In Vivo Application: In vivo Blockade of experimental atopic dermatitis via topical NF-kappaB decoy oligonucleotide. Authors: Dajee M, Muchamuel T, Schryver B, Oo A, Alleman-Sposeto J, De Vry CG, Prasad S, Ruhrmund D, Shyamsundar R, Mutnick D, Mai K, Le T, Parham C, Zhang J, Komuves L, Colby T, Hudak S, McEvoy LM, Ehrhardt RO J. Invest. Dermatol., 2006;126(8):1792-803. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice. Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG Inflamm. Res., 2006;55(11):457-64. Species: Mouse Sample Type: Synovial Fluid Application: ELISA Development Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG J. Immunol., 2006;177(3):1967-74. Species: Mouse Sample Type: Plasma Application: ELISA Development Sequential ELISA to profile multiple cytokines from small volumes. Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG J. Immunol. Methods, 2005;302(1):172-81. Species: Mouse Sample Type: Plasma Application: ELISA Development Selective macrophage suppression during sepsis. Authors: Ellaban E, Bolgos G, Remick D Cell. Immunol., 2004;231(1):103-11. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant mouse IL-1 beta /IL-1F2 Val118-Ser269 Accession # P10749
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65-78% identity with canine, equine, feline, human, porcine, and rhesus macaque IL‑1 beta.
运输条件	Blue Ice
存放说明	4℃
参考文献	Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. Gray, P.W. et al. (1986) J. Immunol. 137:3644. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

参考图片

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB401). The ND₅₀ is typically 1-5 µg/mL in the presence of concanavalin A (1.25 µg/mL).