Human IL-4 MAb (Clone 3007) (25 UG)【科瑞奥博】

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Human IL-4 MAb (Clone 3007) (25 UG)

货号： MAB304-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB304-SP
别名	B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra
全称	Interleukin 4
反应种属	Human
应用	Western Blot(1 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects human IL-4 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑4.
使用方法	Western Blot: 1 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.03-0.1 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.
来源	Monoclonal Mouse IgG₁ Clone # 3007
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The long intergenic noncoding RNA landscape of human lymphocytes highlights the regulation of T cell differentiation by linc-MAF-4. Authors: Ranzani, Valeria, Rossetti, Grazisa, Panzeri, Ilaria, Arrigoni, Alberto, Bonnal, Raoul J, Curti, Serena, Gruarin, Paola, Provasi, Elena, Sugliano, Elisa, Marconi, Maurizio, De Francesco, Raffaele, Geginat, Jens, Bodega, Beatrice, Abrignani, Sergio, Pagani, Massimil Nat Immunol, 2015;16(3):318-25. Species: Human Sample Type: Whole Cells Application: Neut IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A J. Immunol., 2010;184(9):4880-8. Species: Human Sample Type: Whole Cells Application: Neut Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema. Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH J. Allergy Clin. Immunol., 2007;120(5):1118-25. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Interleukin-4 induces human hepatocyte apoptosis through a Fas-independent pathway. Authors: Aoudjehane L, Podevin P, Scatton O, Jaffray P, Dusanter-Fourt I, Feldmann G, Massault PP, Grira L, Bringuier A, Dousset B, Chouzenoux S, Soubrane O, Calmus Y, Conti F FASEB J., 2007;21(7):1433-44. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL‑4 His25-Ser153 Accession # P05112.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5-7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on non-hematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10-13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. Redfield, C. et al. (1991) Biochemistry 30:11029. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451. Mosman, T.R. et al. (1987) J. Immunol. 138:1813. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701. Paludan, S.R. (1998) Scand. J. Immunol. 48:459. Corthay, A. (2006) Scand. J. Immunol. 64:93. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

参考图片

IL‑4 in Human PBMCs. IL‑4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB304). The ND₅₀ is typically 0.03-0.1 µg/mL.