| 货号 | MAB212-SP |
| 别名 | Astrocyte-derived trophic factor; ATF; ATF1; ATF2; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3 | 全称 | Glial Cell line-derived Growth Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects human GDNF in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta -NGF, rhNT-3, or rhNT-4. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of Recombinant Human GFR alpha -1/GDNF R alpha -1 Fc Chimera and 40 ng/mL Recombinant Human GDNF. |
| 来源 | Monoclonal Mouse IgG1 Clone # 27106 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2668 (Human); 25453 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. PSA-NCAM-dependent GDNF signaling limits neurodegeneration and epileptogenesis in temporal lobe epilepsy. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human GDNF Arg109-Ile211 Accession # P39905 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Proliferation Induced by GDNF and Neutralization by Human GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by the Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDNF Monoclonal Antibody (Catalog # MAB212). The ND50 is typically 0.4‑2 μg/mL. |
