Human VEGF MAb (Clone 26503) (25 UG)【科瑞奥博】

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Human VEGF MAb (Clone 26503) (25 UG)

货号： MAB293-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB293-SP
别名	MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor
全称	Vascular Endothelial Growth Factor
反应种属	Human
应用	Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects human VEGF₁₆₅ and human VEGF₁₂₁ in ELISAs and Western blots. In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and rrVEGF and no cross-reactivity with rhVEGF-D.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize VEGF₁₆₅-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.04-0.08 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF₁₆₅.
来源	Monoclonal Mouse IgG_2B Clone # 26503
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang PLoS ONE, 2016;11(4):e0152789. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis. Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T J Biol Chem, 2015;290(36):22212-24. Species: Mouse Sample Type: In Vivo Application: Neut Pericytes contribute to the disruption of the cerebral endothelial barrier via increasing VEGF expression: implications for stroke. Authors: Bai Y, Zhu X, Chao J, Zhang Y, Qian C, Li P, Liu D, Han B, Zhao L, Zhang J, Buch S, Teng G, Hu G, Yao H PLoS ONE, 2015;10(4):e0124362. Species: Mouse Sample Type: In Vivo Application: In vivo Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis. Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A Nat Commun, 2014;5(0):4715. Species: Human Sample Type: In Vivo Application: Neut Heat shock protein 27 attenuates neointima formation and accelerates reendothelialization after arterial injury and stent implantation: importance of vascular endothelial growth factor up-regulation. Authors: Ma X, Hibbert B, McNulty M, Hu T, Zhao X, Ramirez F, Simard T, de Belleroche J, OBrien E FASEB J, 2014;28(2):594-602. Species: Human Sample Type: Whole Cells Application: Neut Markers of T cell infiltration and function associate with favorable outcome in vascularized high-grade serous ovarian carcinoma. Authors: Townsend, Katelin, Spowart, Jaeline, Huwait, Hassan, Eshragh, Sima, West, Nathan R, Elrick, Mary A, Kalloger, Steve E, Anglesio, Michael, Watson, Peter H, Huntsman, David G, Lum, Julian J PLoS ONE, 2013;8(12):e82406. Species: Human Sample Type: tissue Application: IHC Paraffin-embedded Hematopoietic stem cell cytokines and fibroblast growth factor-2 stimulate human endothelial cell-pericyte tube co-assembly in 3D fibrin matrices under serum-free defined conditions. Authors: Smith A, Bowers S, Stratman A, Davis G PLoS ONE, 2013;8(12):e85147. Species: Human Sample Type: Whole Cells Application: Neut The transcription factor E2F1 and the SR protein SC35 control the ratio of pro-angiogenic versus antiangiogenic isoforms of vascular endothelial growth factor-A to inhibit neovascularization in vivo. Authors: Merdzhanova G, Gout S, Keramidas M, Edmond V, Coll JL, Brambilla C, Brambilla E, Gazzeri S, Eymin B Oncogene, 2010;29(39):5392-403. Species: Human Sample Type: Cell Culture Supernates Application: WB Complementary actions of inhibitors of angiopoietin-2 and VEGF on tumor angiogenesis and growth. Authors: Hashizume H, Falcon BL, Kuroda T, Baluk P, Coxon A, Yu D, Bready JV, Oliner JD, McDonald DM Cancer Res., 2010;70(6):2213-23. Species: Human Sample Type: In Vivo Application: In vivo A small-molecule triptolide suppresses angiogenesis and invasion of human anaplastic thyroid carcinoma cells via down-regulation of the nuclear factor-kappa B pathway. Authors: Zhu W, Ou Y, Li Y, Xiao R, Shu M, Zhou Y, Xie J, He S, Qiu P, Yan G Mol. Pharmacol., 2009;75(4):812-9. Species: Human Sample Type: Cell Lysates Application: WB Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development FGF5 as an oncogenic factor in human glioblastoma multiforme: autocrine and paracrine activities. Authors: Allerstorfer S, Sonvilla G, Fischer H, Spiegl-Kreinecker S, Gauglhofer C, Setinek U, Czech T, Marosi C, Buchroithner J, Pichler J, Silye R, Mohr T, Holzmann K, Grasl-Kraupp B, Marian B, Grusch M, Fischer J, Micksche M, Berger W Oncogene, 2008;27(30):4180-90. Species: Human Sample Type: Whole Cells Application: Neut Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Authors: Ball SG, Shuttleworth CA, Kielty CM J. Cell Biol., 2007;177(3):489-500. Species: Human Sample Type: Cell Lysates Application: WB Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment. Authors: Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA Blood, 2007;109(1):122-9. Species: Human Sample Type: Cell Culture Supernates Application: Neut Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice. Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K Cancer Res., 2006;66(13):6699-707. Species: Human tumors in Mouse Sample Type: In Vivo Application: In vivo Increased vascular endothelial growth factor and receptors: relationship to angiogenesis in asthma. Authors: Feltis BN, Wignarajah D, Zheng L, Ward C, Reid D, Harding R, Walters EH Am. J. Respir. Crit. Care Med., 2006;173(11):1201-7. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term. Authors: Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ Clin. Sci., 2006;110(5):575-85. Species: Human Sample Type: Tissue Homogenates Application: WB Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential. Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T Am. J. Pathol., 2006;169(4):1167-82. Species: Human Sample Type: Whole Cells Application: Neut Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O Int. J. Cancer, 2006;119(10):2313-21. Species: Human Sample Type: Whole Cells Application: Neut Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas. Authors: Shikada Y, Yonemitsu Y, Koga T, Onimaru M, Nakano T, Okano S, Sata S, Nakagawa K, Yoshino I, Maehara Y, Sueishi K Cancer Res., 2005;65(16):7241-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo. Authors: Li L, Lin X, Staver M, Shoemaker A, Semizarov D, Fesik SW, Shen Y Cancer Res., 2005;65(16):7249-58. Species: Human Sample Type: Whole Tissue Application: IHC Functional and structural remodeling of the myocardial microvasculature in early experimental hypertension. Authors: Rodriguez-Porcel M, Zhu XY, Chade AR, Amores-Arriaga B, Caplice NM, Ritman EL, Lerman A, Lerman LO Am. J. Physiol. Heart Circ. Physiol., 2005;290(3):H978-84. Species: Porcine Sample Type: Whole Tissue Application: IHC Frozen IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H J. Immunol., 2005;175(9):6177-89. Species: Human Sample Type: Cell Culture Supernates Application: Neut Possible mechanisms inducing glomerulations in interstitial cystitis: relationship between endoscopic findings and expression of angiogenic growth factors. Authors: Tamaki M, Saito R, Ogawa O, Yoshimura N, Ueda T J. Urol., 2004;172(3):945-8. Species: Human Sample Type: Whole Cells Application: ICC Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Authors: Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GY Eur. J. Clin. Invest., 2003;33(10):883-90. Species: Human Sample Type: Plasma Application: ELISA Development Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3. Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC Cancer Res., 2003;63(4):887-92. Species: Human Sample Type: Whole Cells Application: Flow Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome. Authors: Zhou Y, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ Am. J. Pathol., 2002;160(4):1405-23. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts. Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M Transplant. Proc., 2000;32(5):987-9. Species: Porcine Sample Type: Whole Tissue Application: IHC Paraffin-embedded Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells. Authors: Gu JW, Adair TH Am. J. Physiol., 1997;273(2):H628-33. Species: Human Sample Type: Cell Culture Supernates Application: Neut VEGF in the lung: a role for novel isoforms. Authors: Varet, Julia, Varet J, Douglas SK, Douglas, Samantha, Gilmartin, Laura, Gilmartin L, Medford, Andrew R, Bates, David O, Harper, Steven J, Millar, Ann B Am J Physiol Lung Cell Mol Physiol, 0;298(6):L768-74. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF₁₆₅ Ala27-Arg191 Accession # NP_001165097.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	VEGF is a soluble protein secreted by a wide variety of cell types. Itbinds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2(Flk-1). VEGF stimulates vascular endothelial cell proliferation and isa potent inducer of angiogenesis. Several VEGF isoforms occur resultingfrom alternative mRNA splicing.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Adult Neurogenesis Airway Inflammation and Remodeling ALS Related Molecules BIObrief: Neuroprotective & Pathogenic Effects of VEGF Signaling Cancer Biomarkers Developmental Factors Implicated in Neurodegeneration HIF Transcription Factors HIF-1 & Vascular Development ICC / IHC Images: VEGF Inflammatory Mediators Macrophage Activation Markers Mini-review: Vascular Endothelial Growth Factor (VEGF) Myeloid-derived Suppressor Cells (MDSC) New and Improved TNF Superfamily Proteins 2015 Platelets Preeclampsia Proliferation Bioassay HUVECs Regulation of VE-Cadherin and VEGF R2 by VEGF Technical Notes: Serum Levels of Vascular Growth Factors During Pregnancy VEGF & Amyotrophic Lateral Sclerosis VEGF Family VEGF Family Ligands VEGF, Angiopoietin-2 and Tumor Vascularization

参考图片

Cell Proliferation Induced by VEGF₁₆₅ and Neutralization by Human VEGF Antibody. Recombinant Human VEGF₁₆₅(Catalog #293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF₁₆₅ (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti- Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND₅₀ is typically 0.04-0.08 µg/mL.

VEGF in Human Liver. VEGF was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.