Human IFN-gamma MAb (Clone 25723) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human IFN-gamma MAb (Clone 25723) (25 UG)

货号： MAB2851-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2851-SP
别名	IFG; IFI; IFNG; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
全称	Interferon gamma
反应种属	Human
应用	Immunocytochemistry(8-25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed. Neutralizes the bioactivity of recombinant human IFN-gamma and will not neutralize the activity of rat or mouse IFN-gamma.
使用方法	Immunocytochemistry: 8-25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 5 ng/mL Recombinant Human IFN‑ gamma.
来源	Monoclonal Mouse IgG_2B Clone # 25723
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23. Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB Exp. Dermatol., 2004;13(12):764-72. Species: Human Sample Type: Whole Tissue Application: IHC Fresh/Frozen
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IFN-gamma Gln24-Gln166 Accession # AAP20098.1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
运输条件	Blue Ice
存放说明	4℃
参考文献	Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97. Pestka, S. et al. (2004) Immunol. Rev. 202:8. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5401. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

参考图片

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # MAB2851). The ND₅₀ is typically 0.2‑0.6 µg/mL.