Human FGF-3 MAb (Clone 254625) (25 UG)【科瑞奥博】

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Human FGF-3 MAb (Clone 254625) (25 UG)

货号： MAB1206-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1206-SP
别名	fibroblast growth factor 3 (murine mammary tumor virus integration site (v-int-2) oncogene homolog); fibroblast growth factor 3; HBGF-3; Hst; INT-2; mouse; oncogene INT2
全称	Fibroblast Growth Factor 3
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(8-25 µg/mL)
目标/特异性	Detects human FGF-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FGF-4, -5, -6, -7, -9, -10, -11, -12, -16, -17, -18, -19, -20, -21, -23, rhFGF acidic, rhFGF basic, recombinant mouse (rm) FGF-8b, -8c, or -15 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize FGF‑3-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 2-5 µg/mL in the presence of 0.3 µg/mL Recombinant Human FGF‑3 and 1 µg/mL heparin.
来源	Monoclonal Mouse IgG_2A Clone # 254625
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2248 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fractional CO2 laser: a novel therapeutic device upon photobiomodulation of tissue remodeling and cytokine pathway of tissue repair. Authors: Prignano F, Campolmi P, Bonan P Dermatol Ther, 2009;22(0):S8-15. Species: Human Sample Type: Whole Tissue Application: IHC
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human FGF-3 Asp28-Arg212 Accession # P11487
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Fibroblast Growth Factor 3 (FGF-3) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4). TheFGF-3 gene, originally designated int-2, was first identified as a proto-oncogene activated in mouse mammary tumors by proviral integration (2). Amplification of this gene has also been found frequently in human tumors. Human FGF-3 cDNA predicts a 239 aa precursor protein with a 17 aa signal peptide and a 222 aa secreted mature protein with one potential N-linked glycosylation site (1). Human and mouse FGF-3 share 88% aa sequence identity. The Xenopus and mammalian secreted FGF-3 are processed proteolytically at both the N- and C-terminus (5). FGF-3 binds with high-affinity to the IIIb isoforms of FGF R1 and FGF R2. FGF-3 also binds the IIIc isoform of FGF R2, but with lower affinity (6). FGF-3 has been implicated in the induction of inner ear development (7). Studies have suggested that FGF-3 and FGF-8 function synergistically in otic placode induction and during neuronal development to regulate dorsoventral axis formation (8 - 10). During development, the activities of FGF-3 and FGF-8 are regulated negatively by the sprouty family proteins and by Sef (similarexpression to fgf genes), a transmebrane protein that shares intracellular sequence similarities with the IL-17 receptor (10).
运输条件	Blue Ice
存放说明	4℃
参考文献	Brookes, S. et al. (1989) Oncogene 4:429. Dickson, C. et al. (1989) Prog. Growth Factor Res. 1:123. Goldfarb, M. (1996) Cytokine and Growth Factor Reviews 7:311. Green, P. et al. (1996) BioEssays 18:639. Antoine, M. et al. (2000) Cell Growth Differen. 11:593. Kohl, R. et al. (2002) J. Biol. Chem. 277:32760. Represa, J. et al. (1991) Nature 353:561. Maroon, H. et al. (2002) Development, 129:2099. Walshe, J. et al. (2002) Current Biol. 12:1117. Furthauer, M. et al. (2002) Nature Cell Biol. 4:170.

参考图片

Cell Proliferation Induced by FGF‑3 and Neutralization by Human FGF‑3 Antibody. Recombinant Human FGF‑3 (Catalog # 1206-F3) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑3 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF‑3 Monoclonal Antibody (Catalog # MAB1206). The ND₅₀ is typically 2-5 µg/mL in the presence of heparin (1 µg/mL).