Human IL-10 MAb (Clone 25209) (25 UG)【科瑞奥博】

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Human IL-10 MAb (Clone 25209) (25 UG)

货号： MAB2171-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2171-SP
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Human
应用	Western Blot(5 µg/mL)
目标/特异性	Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
使用方法	Western Blot: 5 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND₅₀) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.
来源	Monoclonal Mouse IgG_2B Clone # 25209
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. Authors: Svajger U, Obermajer N, Jeras M J Leukoc Biol, 2014;95(1):33-46. Species: Human Sample Type: Whole Cells Application: Neut Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. Authors: Nicolaidou V, Wong MM, Redpath AN PLoS ONE, 2012;7(7):e39871. Species: Human Sample Type: Whole Cells Application: Neut DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function. Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M J. Leukoc. Biol., 2011;89(6):893-905. Species: Human Sample Type: Whole Cells Application: Neut Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM J. Immunol., 2009;182(5):2929-38. Species: Mouse Sample Type: Whole Cells Application: Neut Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Flow Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S J. Immunol., 2007;179(4):2359-67. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL-10 Ser19-Asn178 Accession # P22301
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-10 is a pleiotropic cytokine that can exert either immunostimulatoryor immunosuppressive effects on a variety of cell types. It is producedpredominantly by Th2 lymphocytes and other activated lymphoid cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation B Cell Markers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells ICC / IHC Images: IL-10 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-10/Interferon Family Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Lymphoma Macrophage Activation Markers Monocyte Markers Mouse Obesity Antibody Array Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neuroinflammation Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th22 Cells Th9 Cells

参考图片

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody.

Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171). The ND₅₀ is typically 0.005‑0.03 µg/mL.