Human CCL2/MCP-1 MAb (Clone 24822) (25 UG)【科瑞奥博】

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Human CCL2/MCP-1 MAb (Clone 24822) (25 UG)

货号： MAB279-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB279-SP
别名	MCAF; MCP-1
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CCL2/JE/MCP-1 in Western blots. Does not cross-react with recombinant human (rh) CCL1, 3, 4,5, 7, 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, rmCCL1, 2, 3, 4, 5,6, 7, 9/10MIP-1 gamma, 11, 12, 17, 19, 20, 22, 24, 25, or rrCCL20.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 0.9-4.5 µg/mL in the presence of 0.15 µg/mL Recombinant Human CCL2/JE/MCP‑1.
来源	Monoclonal Mouse IgG₁ Clone # 24822
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. Species: Human Sample Type: Whole Cells Application: Neut Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells. Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N Int. J. Cancer, 2010;126(5):1095-108. Species: Human Sample Type: Whole Cells Application: Neut Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways. Authors: Renaud SJ, Sullivan R, Graham CH Placenta, 2009;30(4):313-9. Species: Human Sample Type: Whole Cells Application: Neut Pre-eclampsia is associated with dendritic cell recruitment into the uterine decidua. Authors: Huang SJ, Chen CP, Schatz F, Rahman M, Abrahams VM, Lockwood CJ J. Pathol., 2008;214(3):328-36. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM Atherosclerosis, 2007;192(1):56-66. Species: Human Sample Type: Whole Cells Application: Neut Eotaxin-2 and colorectal cancer: a potential target for immune therapy. Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE Clin. Cancer Res., 2007;13(19):5719-28. Species: Human Sample Type: Tissue Homogenates Application: ELISA Development Induction of host chemotactic response by Encephalitozoon spp. Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H Infect. Immun., 2007;75(4):1619-25. Species: Human Sample Type: Whole Cells Application: Neut Systemic inflammation in nonalcoholic fatty liver disease is characterized by elevated levels of CCL2. Authors: Haukeland JW, Damas JK, Konopski Z, Loberg EM, Haaland T, Goverud I, Torjesen PA, Birkeland K, Bjoro K, Aukrust P J. Hepatol., 2006;44(6):1167-74. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors. Authors: Penninckx F, Ectors N Exp. Cell Res., 2005;303(2):331-42. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells. Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P J. Leukoc. Biol., 2004;75(3):504-14. Species: Human Sample Type: Application: Flow The CC chemokine ligand, CCL2/MCP1, participates in macrophage fusion and foreign body giant cell formation. Authors: Kyriakides TR, Foster MJ, Keeney GE, Tsai A, Giachelli CM, Clark-Lewis I, Rollins BJ, Bornstein P Am. J. Pathol., 2004;165(6):2157-66. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human CCL2/JE/MCP-1
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL2 is a beta -chemokine that is also known as monocyte chemotacticprotein 1 (MCP-1). It is produced by a variety of cell types and ischemotactic for monocytes.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis gamma delta T Cells HIF Transcription Factors ICC / IHC Images: CCL2/JE/MCP-1 Inflammatory Mediators Macrophage Activation Markers Neuroinflammation

参考图片

Chemotaxis Induced by CCL2/MCP‑1 and Neutralization by Human CCL2/MCP‑1 Antibody. Recombinant Human CCL2/MCP‑1 (Catalog #
279-MC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP‑1 (0.15 µg/mL) is neutralized (green line) by increasing concentrations of
Mouse Anti-Human
CCL2/MCP‑1 Monoclonal Antibody (Catalog # MAB279). The ND₅₀ is typically
0.9-4.5 µg/mL.