Human IL-29/IFN-lambda 1 MAb (Clone 247801) (25 UG)【科瑞奥博】

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Human IL-29/IFN-lambda 1 MAb (Clone 247801) (25 UG)

货号： MAB15981-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB15981-SP
别名	cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21
全称	Interleukin 29
反应种属	Human
应用	ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human IL-29/IFN-lambda 1 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) IL-28B/IFN-lambda 3 and 20% with rhIL‑28A is observed. No cross-reactivity or interference was observed with rhIFN-alpha A, rhIFN-alpha B2, rhIFN-alpha G, rhIFN-alpha I, or rhIFN‑ beta 1A.
使用方法	ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑29/IFN‑ lambda 1 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑29/IFN‑ lambda 1.
来源	Monoclonal Mouse IgG_2A Clone # 247801
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	282618 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Induction and Antagonism of Antiviral Responses in Respiratory Syncytial Virus-Infected Pediatric Airway Epithelium. Authors: Villenave R, Broadbent L, Douglas I, Lyons J, Coyle P, Teng M, Tripp R, Heaney L, Shields M, Power U J Virol, 2015;89(24):12309-18. Species: Human Sample Type: Whole Cells Application: Neut Hepatitis C virus pathogen associated molecular pattern (PAMP) triggers production of lambda-interferons by human plasmacytoid dendritic cells. Authors: Stone, Amy E L, Giugliano, Silvia, Schnell, Gretja, Cheng, Linling, Leahy, Katelyn, Golden-Mason, Lucy, Gale, Michael, Rosen, Hugo R PLoS Pathog, 2013;9(4):e1003316. Species: Human Sample Type: whole cells Application: Neut Type III IFNs are produced by and stimulate human plasmacytoid dendritic cells. Authors: Yin Z, Dai J, Deng J, Sheikh F, Natalia M, Shih T, Lewis-Antes A, Amrute S, Garrigues U, Doyle S, Donnelly R, Kotenko S, Fitzgerald-Bocarsly P J Immunol, 2012;189(6):2735-45. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL-29/IFN-lambda 1 Gly20-Thr200 Accession # Q8IU54
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% aa sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Vilcek, J. (2003) Nature Immunol. 4:8. Sheppard, P. et al. (2003) Nature Immunol. 4:63. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

参考图片

IL‑29/IFN‑ lambda 1 Inhibition of EMCV‑induced Cytopathy and Neutralization by Human IL‑29/IFN‑ lambda 1 Antibody.

Recombinant Human IL‑29/IFN‑ lambda 1 (Catalog # 1598‑IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL‑29/IFN‑ lambda 1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anri-Human IL‑29/IFN‑ lambda 1 Monoclonal Antibody (Catalog # MAB15981). The ND₅₀ is typically 1-4 µg/mL.