Mouse CXCR4 MAb (Clone 247506) (25 UG)【科瑞奥博】

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Mouse CXCR4 MAb (Clone 247506) (25 UG)

货号： MAB21651-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB21651-SP
别名	CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
反应种属	Mouse
应用	Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(8-25 µg/mL) Immunocytochemistry(8-25 µg/mL)
目标/特异性	Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 8-25 µg/mL Immunocytochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR4. The Neutralization Dose (ND₅₀) is typically 8‑40 µg/mL in the presence of 10 ng/mL Recombinant Mouse CXCL12/SDF‑1 alpha.
来源	Monoclonal Rat IgG_2B Clone # 247506
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways Sci Rep, 2016;6(0):25104. Species: Mouse Sample Type: Whole Cells Application: IHC Not Specified CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4. Authors: Chen Q, Zhang M, Li Y, Xu D, Wang Y, Song A, Zhu B, Huang Y, Zheng J Stem Cells, 2015;33(8):2574-85. Species: Mouse Sample Type: Whole Cells Application: IHC - PFA fixed Protein z exerts pro-angiogenic effects and upregulates CXCR4. Authors: Butschkau, Antje, Wagner, Nana-Mar, Genz, Berit, Vollmar, Brigitte PLoS ONE, 2014;9(12):e113554. Species: Human Sample Type: Whole Cells Application: ICC Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo. Authors: Kavanagh D, Yemm A, Zhao Y, Frampton J, Kalia N PLoS ONE, 2013;8(3):e59150. Species: Mouse Sample Type: Whole Cells Application: Flow MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1. Authors: Salvucci O, Jiang K, Gasperini P Haematologica, 2012;97(6):818-26. Species: Mouse Sample Type: Whole Cells Application: Flow Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration. Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I Eur. J. Immunol., 2012;42(5):1226-36. Species: Mouse Sample Type: Whole Cells Application: Flow The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury. Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X PLoS ONE, 2012;7(4):e34608. Species: Mouse Sample Type: Whole Cells Application: Neut IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation. Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN J. Immunol., 2011;187(9):4835-43. Species: Mouse Sample Type: Whole Cells Application: Flow Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs. Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X Biochem. Biophys. Res. Commun., 2010;401(4):509-15. Species: Mouse Sample Type: Whole Cells Application: Neut Paracrine modulation of CXCR4 by IGF-1 and VEGF: implications for choroidal neovascularization. Authors: Sengupta N, Afzal A, Caballero S, Chang KH, Shaw LC, Pang JJ, Bond VC, Bhutto I, Baba T, Lutty GA, Grant MB Invest. Ophthalmol. Vis. Sci., 2010;51(5):2697-704. Species: Mouse Sample Type: In Vivo Application: In vivo Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching. Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M Blood, 2010;115(24):5102-10. Species: Mouse Sample Type: In Vivo Application: In vivo Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice. Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S Atherosclerosis, 2009;205(1):63-73. Species: Mouse Sample Type: Whole Tissue Application: IHC Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells. Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL Cancer Res., 2008;68(6):1889-95. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells. Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV J. Cell Biol., 2008;183(6):1145-57. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Dual effect of AMD3100, a CXCR4 antagonist, on bleomycin-induced lung inflammation. Authors: Watanabe M, Matsuyama W, Shirahama Y, Mitsuyama H, Oonakahara K, Noma S, Higashimoto I, Osame M, Arimura K J. Immunol., 2007;178(9):5888-98. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Y3 rat myeloid cell line transfected with mouse CXCR4 Met1-Ser359 Accession # P70658
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Myeloid Leukemia B Cell Chemotaxis, Migration, and Adhesion B Cell Markers Cancer Biomarkers Cancer Stem Cell Markers Chemokine Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Chemokines and Receptors in Tfh Cell Differentiation and Function CXCR4: A Receptor for Extracellular Ubiquitin Cytokine Upregulation of HIV Coreceptors Endothelial Cell Differentiation Markers Endothelial Progenitor and Endothelial Cell Markers Endothelial Progenitor Cell Markers gamma delta T Cells Hematopoietic Stem Cell Markers Hepatic Endoderm Hepatic Endoderm Cell Markers HIF Transcription Factors Human CD Marker Products Infectious Diseases Megakaryocytes Neural Stem Cell Markers Neuroinflammation Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) Somite Markers T Cell Migration/Adhesion Th2 Cells

参考图片

Chemotaxis Induced by CXCL12/SDF‑1 alpha and Neutralization by Mouse CXCR4 Antibody. Recombinant Mouse CXCL12/SDF‑1 alpha (Catalog # 460-SD) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND₅₀ is typically 8-40 µg/mL.

CXCR4 in Mouse Splenocytes. CXCR4 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CXCR4 in Mouse Neural Progenitor Cells. CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CXCR4 in Mouse Spleen. CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.