| 货号 | MAB25451-SP |
| 别名 | Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI-2REF1; tissue factor pathway inhibitor 2 | 全称 | Tissue Factor Pathway Inhibitor 2 |
| 反应种属 | Human |
| 应用 | Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human TFPI‑2 in direct ELISAs and Western blots. |
| 使用方法 | Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human TFPI-2 (0.017 µg/mL, Catalog # 2545-PI) inhibition of Trypsin (0.025 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 0.45 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 243227 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7980 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Syncytiotrophoblast microvesicles released from pre-eclampsia placentae exhibit increased tissue factor activity. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human TFPI‑2 Asp23-Phe235 Accession # P48307 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains (residues 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4). The purified Recombinant Human TFPI-2 ends at residue 213 and does not contain the last 22 residues (residues 214 to 235) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Tissue Factor (Catalog # 2339-PA). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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