Mouse CXCR2 MAb (Clone 242216) (25 UG)【科瑞奥博】

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Mouse CXCR2 MAb (Clone 242216) (25 UG)

货号： MAB2164-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB2164-SP
别名	beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
全称	Interleukin 8 Receptor B
反应种属	Mouse
应用	Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Stains mouse CXCR2 transfectedcells but not CXCR1 transfectants.
使用方法	Flow Cytometry: 0.25 µg/10⁶cells Neutralization: Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR2. The Neutralization Dose (ND₅₀) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.
来源	Monoclonal Rat IgG_2A Clone # 242216
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3579 (Human); 12765 (Mouse); 29385 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy. Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K J Biol Chem, 2015;290(4):2053-68. Species: Mouse Sample Type: Whole Tissue Application: IHC The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation. Authors: Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G Mol Cell Biol, 2015;35(3):598-609. Species: Mouse Sample Type: In Vivo Application: Neut IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming. Authors: Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R J Immunol, 2014;192(2):704-13. Species: Mouse Sample Type: Whole Cells Application: Flow CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta. Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S Int J Cancer, 2014;134(6):1346-58. Species: Mouse Sample Type: In Vivo Application: Neut CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis. Authors: Roy M, Richard JF, Dumas A, Vallieres L J Neuroinflammation, 2012;9(0):18. Species: Mouse Sample Type: In Vivo Application: In vivo TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG J. Lipid Res., 2011;52(7):1307-18. Species: Mouse Sample Type: In Vivo Application: In vivo Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice. Authors: von Vietinghoff S, Asagiri M, Azar D J. Immunol., 2010;185(1):670-8. Species: Mouse Sample Type: In Vivo Application: In vivo Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice. Authors: Traylor ZP, Yu EN, Davis IC Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L437-45. Species: Mouse Sample Type: In Vivo Application: In vivo Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1. Authors: Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS Am. J. Pathol., 2009;174(1):164-76. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9. Species: Mouse Sample Type: Whole Cells Application: Neut An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition. Authors: Nomellini V, Faunce DE, Gomez CR, Kovacs EJ J. Leukoc. Biol., 2008;83(6):1493-501. Species: Mouse Sample Type: In Vivo Application: In vivo HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM Blood, 2008;111(7):3343-54. Species: Mouse Sample Type: Whole Cells Application: Neut Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice. Authors: Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K Blood, 2008;111(12):5457-66. Species: Mouse Sample Type: Whole Cells Application: Flow Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice. Authors: Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(3):L753-61. Species: Mouse Sample Type: Whole Tissue Application: IHC An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Authors: Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL Blood, 2007;109(9):3794-802. Species: Mouse Sample Type: Whole Cells Application: Flow Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice. Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T Eur. J. Immunol., 2007;37(10):2753-63. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	C6 rat glioma cell line transfected with mouse CXCR2 Met1-Leu359 Accession # P35343
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.
运输条件	Blue Ice
存放说明	4℃
参考文献	Chemokine Receptors Chemokines and Receptors in Angiogenesis Human CD Marker Products Macrophage Activation Markers Megakaryocytes Neuroinflammation Neutrophils T Cell Migration/Adhesion

参考图片

Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. Recombinant Mouse CXCL2/MIP‑2 (Catalog #
452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164). The ND₅₀ is typically 15-50 µg/mL.

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse
CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).