Human IL-10 MAb (Clone 23738) (25 UG)【科瑞奥博】

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Human IL-10 MAb (Clone 23738) (25 UG)

货号： MAB217-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB217-SP
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Human
应用	Western Blot(2 µg/mL)
目标/特异性	Detects human IL-10 in Western blots. In Western blots, this antibody shows approximately 5% cross-reactivity with recombinant mouse IL-10.
使用方法	Western Blot: 2 µg/mL Simple Western: 10 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. At 2 µg/mL, this antibody will neutralize >60% of the bioactivity due to 5 ng/mL Recombinant Human IL‑10.
来源	Monoclonal Mouse IgG_2B Clone # 23738
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages Sci Rep, 2016;6(0):31959. Species: Human Sample Type: Whole Cells Application: Blocking Differential signaling of cmvIL-10 through common variants of the IL-10 receptor 1. Authors: Gruber SG, Gloria Luciani M, Grundtner P, Zdanov A, Gasche C Eur. J. Immunol., 2008;38(12):3365-75. Species: Human Sample Type: recombinant IL-10 Application: WB Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses. Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ J. Virol., 2007;81(11):5882-92. Species: Human Sample Type: Whole Cells Application: Neut Induction of host chemotactic response by Encephalitozoon spp. Authors: Fischer J, West J, Agochukwu N, Suire C, Hale-Donze H Infect. Immun., 2007;75(4):1619-25. Species: Human Sample Type: Whole Cells Application: Neut Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells. Authors: Bayry J, Triebel F, Kaveri SV, Tough DF J. Immunol., 2007;178(7):4184-93. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Neut Increased frequency of CD4+ cells expressing CD161 in cancer patients. Authors: Iliopoulou EG, Karamouzis MV, Missitzis I, Ardavanis A, Sotiriadou NN, Baxevanis CN, Rigatos G, Papamichail M, Perez SA Clin. Cancer Res., 2006;12(23):6901-9. Species: Human Sample Type: Whole Cells Application: Neut Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Authors: Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Blood, 2005;105(2):750-8. Species: Human Sample Type: Cell Culture Supernates Application: Neut Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis. Authors: Phillips TM Electrophoresis, 2004;25(10):1652-9. Species: Human Sample Type: Serum Application: Affinity Chromatography Distinct transcriptional programs activated by interleukin-10 with or without lipopolysaccharide in dendritic cells: induction of the B cell-activating chemokine, CXC chemokine ligand 13. Authors: Perrier P, Martinez FO, Locati M, Bianchi G, Nebuloni M, Vago G, Bazzoni F, Sozzani S, Allavena P, Mantovani A J. Immunol., 2004;172(11):7031-42. Species: Human Sample Type: Whole Cells Application: Neut In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures. Authors: Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB Blood, 2004;104(2):453-61. Species: Human Sample Type: Whole Cells Application: Flow Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Immunology, 2004;112(2):274-89. Species: Human Sample Type: Whole Cells Application: Flow IL-15 and cognate antigen successfully expand de novo-induced human antigen-specific regulatory CD4+ T cells that require antigen-specific activation for suppression. Authors: Koenen HJ, Fasse E, Joosten I J. Immunol., 2003;171(12):6431-41. Species: Human Sample Type: Whole Cells Application: Neut Regulatory activity of autocrine IL-10 on dendritic cell functions. Authors: Corinti S, la Sala A, Pastore S J. Immunol., 2001;166(7):4312-8. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL‑10 Ser19-Asn178 Accession # P22301
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation B Cell Markers Conventional/Classical Dendritic Cells Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function gamma delta T Cells ICC / IHC Images: IL-10 IL-10 and IL-12 Influence in Dendritic Cell Maturation IL-10/Interferon Family Inflammatory Mediators Isolation, Culture, and Identification of Natural Killer Cells Lymphoma Macrophage Activation Markers Monocyte Markers Mouse Obesity Antibody Array Multiple Sclerosis and Myelin Related Molecules Myeloid-derived Suppressor Cells (MDSC) Natural Killer T (NKT) Cells Neuroinflammation Plasmacytoid Dendritic Cells Regulatory T Cells (Tregs) T Cell Cytokine Signaling Th1 Cells Th2 Cells Th22 Cells Th9 Cells

参考图片

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB217). At 5 µg/mL, this antibody will neutralize 60% Recombinant Human IL-10 induced bioactivity.