Canine TNF-alpha MAb (Clone 236812) (25 UG)【科瑞奥博】

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Canine TNF-alpha MAb (Clone 236812) (25 UG)

货号： MAB1507-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1507-SP
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Canine
应用	Immunocytochemistry(8-25 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects canine TNF-alpha in direct ELISAs. In direct ELISAs, 25-100% cross-reactivity with recombinant human TNF-alpha, recombinant porcine TNF-alpha, and recombinant rhesus macaque TNF-alpha is observed.
使用方法	Immunocytochemistry: 8-25 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 1.25-5.0 µg/mL in the presence of 10 ng/mL Recombinant Canine TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 236812
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum. Authors: Figueiredo M, Amorim I, Pinto A, Barbosa V, Pinheiro L, Deoti B, Faria A, Tafuri W BMC Immunol, 2013;14(0):22. Species: Canine Sample Type: Tissue Homogenates Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant canine TNF-alpha Val77-Leu233 Accession # P51742
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Canine
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Canine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, canine TNF-alpha shares 84‑94% aa sequence identity with equine, feline, human, porcine, and rhesus and 69-77% with bovine, cotton rat, mouse, and rat with TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Zucker, K. et al. (1994) Lymphokine Cytokine Res. 13:191. Tang, P. et al. (1996) Biochemistry 35:8216. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. Romanatto, T. et al. (2007) Peptides 28:1050. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Canine TNF‑ alpha Antibody. Recombinant Canine TNF‑ alpha (Catalog #
1507-CT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Canine TNF‑ alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507). The ND₅₀ is typically
1.25-5.0 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

TNF‑ alpha in Canine PBMCs. TNF‑ alpha was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) untreated or treated with calcium ionomycin and PMA using Mouse Anti-Canine TNF‑ alpha Monoclonal Antibody (Catalog # MAB1507) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.