Human TNF RII/TNFRSF1B MAb (Clone 22210) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TNF RII/TNFRSF1B MAb (Clone 22210) (25 UG)

货号： MAB726-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB726-SP
别名	CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
全称	Tumor Necrosis Factor Receptor II
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human TNF RII in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) TNF RI, recombinant mouse (rm) TNF RI, rmTNF RII, rmTNF‑ alpha, rhTNF‑ alpha, or rhTNF‑ beta. In Western blots, this antibody does not cross‑react with rhTNF RI, rmTNF RI, or rmTNF RII.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TNF RII/TNFRSF1B-mediated inhibition of cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.3 µg/mL Recombinant Human TNF RII/TNFRSF1B Fc Chimera, 0.25 ng/mL Recombinant Human TNF‑ alpha, and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 22210
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7133 (Human); 21938 (Mouse); 156767 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Genome-wide comparison between IL-17A- and IL-17F-induced effects in human rheumatoid arthritis synoviocytes. Authors: Zrioual S, Ecochard R, Tournadre A, Lenief V, Cazalis MA, Miossec P J. Immunol., 2009;182(0):3112-20. Species: Human Sample Type: Whole Cells Application: Neut Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells. Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U J. Immunol., 2007;179(6):4239-48. Species: Human Sample Type: Whole Cells Application: Neut Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. Authors: Copeland S, Siddiqui J, Remick D J. Immunol. Methods, 2004;284(1):99-106. Species: Human Sample Type: Cell Culture Supernates Application: Array Development A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis. Authors: Bharucha AE, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD Am. J. Gastroenterol., 2000;95(9):2338-42. Species: Human Sample Type: Serum Application: ELISA Development Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder. Authors: Jyonouchi H, Sun S, Itokazu N Neuropsychobiology, 0;46(2):76-84. Species: Human Sample Type: Whole Cells Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human TNF RII/TNFRSF1B Leu23-Asp257 Accession # P20333
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF RII, also known as p75 or p80 TNF R, is a prototypic member of theTNF receptor superfamily and has been designated TNFRSF1B. It binds TNFwith high affinity and is expressed primarily in cells of the immunesystem.
运输条件	Blue Ice
存放说明	4℃
参考文献	Cell Structure and Signaling Molecules Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy Endothelial Cell Markers Human CD Marker Products ICC / IHC Images: TNF RII/TNFRSF1B Neuroinflammation Obesity Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members Regulatory T Cells (Tregs) T Cell Cytokine Signaling TNF Superfamily TNF Superfamily Receptors

参考图片

TNF RII/TNFRSF1B Inhibition of TNF‑ alpha -induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2) inhibits Recombinant Human TNF‑ alpha (Catalog # 210-TA) induced cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of Recombinant Human TNF‑ alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB726). The ND₅₀ is typically
0.5-1.5 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).