| 货号 | MAB1999-SP |
| 别名 | endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( ) |
| 目标/特异性 | Detects mouse Endocan/ESM‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Endocan is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 0.75-3.5 µg/mL in the presence of 25 µg/mL Recombinant Mouse Endocan/ESM‑1. |
| 来源 | Monoclonal Rat IgG2B Clone # 220008 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 11082 (Human); 71690 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM‑1 Trp20-Arg184 Accession # Q9QYY7 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Endocan (endothelial cell proteoglycan), also known as endothelial-cell specific molecule-1 (ESM-1), is a 50 kDa monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan shares 96% and 74% aa identity with rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa 82-131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 (integrin alpha Lbeta2) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Mouse Endocan/ ESM‑1 Antibody. Recombinant Mouse Endocan/ESM‑1 (Catalog # 1999-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM‑1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Endocan/ESM‑1 Monoclonal Antibody (Catalog # MAB1999). The ND50 is typically 0.75‑3.5 µg/mL. |
