Human CCL5/RANTES MAb (Clone 21418) (25 UG)【科瑞奥博】

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Human CCL5/RANTES MAb (Clone 21418) (25 UG)

货号： MAB678-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB678-SP
别名	chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
反应种属	Human
应用	Western Blot(1 µg/mL) ELISA Capture (Matched Antibody Pair)(2-8 µg/mL ) ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL ) ELISA Standard ( )
目标/特异性	Detects human and primate CCL5/RANTES in direct ELISAs and Western blots. When used in combination with the biotinylated anti-human/primate CCL5/RANTES detection antibody (Catalog # BAF278) in sandwich ELISAs, no significant cross-reactivity or interference is observed with recombinant human (rh) ANG, rhCNTF, rh beta -ECGF, rhEGF, mouse (m) EGF, rhEpo, bovine (b) FGF acidic, rhFGF acidic, bFGF basic, rhFGF basic, rhFGF-4, rhG-CSF, rhGM-CSF, recombinant mouse (rm) GM-CSF, rhGRO alpha, rhIFN-gamma, rhIGF-I, rhIGF-II, rhIL-1 alpha, rmIL-1 alpha, rhIL-1 beta, rmIL‑1 beta, rhIL-1ra, recombinant rat (rr) IL-1ra, rhIL-2, rhIL-3, rmIL-3, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL‑10, rhIL-11, rhLIF, rhMCP‑1, rhM-CSF, rhMIP-1 alpha, rmMIP-1 alpha, rhMIP-1 beta, rmMIP-1 beta, rhOSM, human (h) PDGF, pPDGF, rhPDGF-AA, rhPDGF‑AB, rhPDGF-BB, rhSCF, rmSCF, rhSLPI, rhTGF-alpha, pTGF-beta 1, hTGF-beta 1, rhTGF-beta 1, pTGF-beta 2, rhTGF-beta 2, recombinant canine TGF-beta 3, rhTGF-beta 3, recombinant amphibian TGF-beta 5, rhTNF-alpha, rmTNF-alpha, or rmTNF-beta.
使用方法	Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.04-0.12 µg/mL in the presence of 10 ng/mL Recombinant Human CCL5/RANTES.
来源	Monoclonal Mouse IgG₁ Clone # 21418
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Development and validation of sandwich ELISA microarrays with minimal assay interference. Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA J. Proteome Res., 2008;7(6):2406-14. Species: Human Sample Type: Serum Application: ELISA Microarray Development Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma. Authors: Osei-Kumah A, Smith R, Clifton VL Cytokine, 2008;43(2):187-93. Species: Human Sample Type: Plasma Application: ELISA Development Relation of circulating concentrations of chemokine receptor CCR5 ligands to C-peptide, proinsulin and HbA1c and disease progression in type 1 diabetes. Authors: Pfleger C, Kaas A, Hansen L, Alizadeh B, Hougaard P, Holl R, Kolb H, Roep BO, Mortensen HB, Schloot NC Clin. Immunol., 2008;128(1):57-65. Species: Human Sample Type: Serum Application: ELISA Development Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions. Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A Int. J. Cancer, 2008;122(4):769-76. Species: Human Sample Type: Whole Cells Application: Neut Ultrasensitive flow-based immunoassays using single-molecule counting. Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Clin. Chem., 2007;53(11):1990-5. Species: Human Sample Type: Plasma Application: ELISA Development HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P AIDS, 2007;21(10):1263-72. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Clin. Chem., 2007;53(11):2010-2. Species: Human Sample Type: Plasma Application: ELISA Development Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Blood, 2007;109(10):4272-9. Species: Human Sample Type: Cell Culture Supernates Application: Luminex Development Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression. Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B J. Virol., 2006;80(1):236-45. Species: Primate - Macaca fascicularis (Crab-eating or Cynomolgus macaque) Sample Type: Cell Culture Supernates Application: ELISA Development Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES. Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD Int. Immunol., 2006;18(8):1243-51. Species: Human Sample Type: Cell Culture Supernates Application: Mass Spectometry Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells. Authors: Edwards MR, Johnson MW, Johnston SL Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Transforming growth factor beta 1(TGF-beta1) down-regulates TNFalpha-induced RANTES production in rheumatoid synovial fibroblasts through NF-kappaB-mediated transcriptional repression. Authors: Cho ML, Min SY, Chang SH, Kim KW, Heo SB, Lee SH, Park SH, Cho CS, Kim HY Immunol. Lett., 2006;105(2):159-66. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA J. Immunol., 2002;169(9):5244-51. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain. Authors: Hirst SJ, Hallsworth MP, Peng Q Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA Immunity, 2001;14(6):705-14. Species: Human Sample Type: Cell Culture Supernates Application: ELISA Development Chemoattractant factors in breast milk from allergic and nonallergic mothers. Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP Pediatr. Res., 2000;47(5):592-7. Species: Human Sample Type: Serum Application: ELISA Development
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human CCL5/RANTES
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human, Primate
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a member of the beta (C-C) chemokine subfamily. It binds and activates the chemokine receptors CCR1, 3 and 5.
运输条件	Blue Ice
存放说明	4℃
参考文献	Airway Inflammation and Remodeling CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Cytokines Suppress HIV Replication Eosinophils ICC / IHC Images: CCL5/RANTES Infectious Diseases Macrophage Activation Markers Neuroinflammation Platelets

参考图片

Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody. Recombinant Human CCL5/RANTES (Catalog #
278‑RN) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate CCL5/RANTES Monoclonal Antibody (Catalog # MAB678). The ND₅₀ is typically 0.04-0.12 µg/mL.