| 货号 | MAB17581-SP |
| 别名 | Rae1; Rae-1 | 全称 | Retinoic Acid Early Transcript 1 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse Rae‑1 alpha / beta /δ/ epsilon. When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma. |
| 使用方法 | Flow Cytometry: 2.5 µg/106cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-6 ng/mL of this antibody will block 50% of the binding of 300 ng/mL of Biotinylated Recombinant Mouse NKG2D Fc Chimera to immobilized Recombinant Mouse Rae-1δ Fc Chimera (Catalog # 1134‑RA) coated at 1 µg/mL (100 µL/well). At 100 ng/mL, this antibody will block >90% of the binding. Blocking of Recombinant Mouse Rae-1 beta is observed at a similar antibody concentration, however, this antibody does not block the binding of Recombinant Mouse NKG2D Fc Chimera to Recombinant Mouse Rae-1 alpha or Rae-1 epsilon in this assay. |
| 来源 | Monoclonal Rat IgG2A Clone # 199205 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8480 (Human); 19368 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58) |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Rae-1 alpha, beta, gamma, δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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