Human CCR8 MAb (Clone 191704) (25 UG)【科瑞奥博】

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Human CCR8 MAb (Clone 191704) (25 UG)

货号： MAB1429-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1429-SP
简介	Clone 191704 was used by HLDA to establish CD designation
别名	CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1
反应种属	Human
应用	Neutralization
目标/特异性	Detects human CCR8. Neutralizes the bioactivity of human CCR8 and specifically stains human CCR8-transfected cell lines but not with the parent cell lines.
使用方法	Neutralization: Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 20 ng/mL Recombinant Human CCL1/I‑309/TCA‑3.
来源	Monoclonal Rat IgG_2B Clone # 191704
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1237 (Human); 12776 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. Authors: Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M, OConnor D, Lifson JD, Roederer M, Ohlen C J. Immunol., 2010;184(1):303-14. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow Distribution, persistence, and efficacy of adoptively transferred central and effector memory-derived autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones in rhesus macaques during acute infection. Authors: Minang JT, Trivett MT, Bolton DL, Trubey CM, Estes JD, Li Y, Smedley J, Pung R, Rosati M, Jalah R, Pavlakis GN, Felber BK, Piatak M, Roederer M, Lifson JD, Ott DE, Ohlen C J. Immunol., 2010;184(1):315-26. Species: Primate - Macaca mulatta (Rhesus monkey) Sample Type: Whole Cells Application: Flow The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors. Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS J. Immunol., 2006;177(7):4488-94. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	BaF3 mouse pro-B cell line transfected with human CCR8 Met1-Leu355 Accession # P51685
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	CCR8 (C-C chemokine receptor 8; previously called CKR-L1 or TER1 and designated CD198) is a41-43 kDa member of the GPCR 1 family of transmembrane proteins. CCR8 is expressed on vascular smoothmuscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+T cells, Langerhans cells and neurons. CCL1/TCA3and vMIP-1 are known agonists for CCR8. Human CCR8 is a 7-transmembrane protein that is 355 amino acids (aa) inlength. It containsa 35 aa N-terminal extracellular domain plus a 51 aa C-terminal cytoplasmic tail. Over aa sequences 1-33 and 92-105collectively, human and mouse CCR8 share 64% aa sequence identity.
运输条件	Blue Ice
存放说明	4℃
参考文献	CC Chemokines and Receptors Chemokine Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Human CD Marker Products Monocyte Markers Regulatory T Cells (Tregs) T Cell Migration/Adhesion Technical Notes: Antibodies to Complex Antigens Th2 Cells Thymocytes

参考图片

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCR8 Antibody.

Recombinant Human CCL1/I‑309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I‑309 (20 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR8 Monoclonal Antibody (Catalog # MAB1429). The ND₅₀ is typically
0.01‑0.05 µg/mL.