| 货号 | MAB1116-SP |
| 别名 | CXCL7/Thymus Chemokine-1; Thymus Chemokine1 |
| 反应种属 | Rat |
| 应用 | Western Blot,ELISA Capture (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects rat CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference with recombinant human CXCL5, recombinant mouse CXCL7/Thymus Chemokine-1, recombinant rat (rr) CXCL1/CINC1, rrCXCL2/CINC3, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, or rrCXCL5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 15 ng/mL Recombinant Rat CXCL7/Thymus Chemokine‑1. |
| 来源 | Monoclonal Mouse IgG2B Clone # 186701 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57349 (Mouse); 246358 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1 Ile46-Ile107 Accession # NP_714943 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CXCL7, also known as Thymus Chemokine-1, is a member of the alpha (CXC) subfamily of chemokines. It functions as a chemoattractant for neutrophils, macrophages and T cells that express CXCR2/IL-8 R beta. Rat CXCL7 cDNA encodes a 111 amino acid (aa) precursor protein. Rat CXCL7 shares 72% aa sequence identity with mouse CXCL7. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Rat CXCL7/Thymus Chemokine‑1 (Catalog # 1116-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/ Thymus Chemokine‑1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL7/ Thymus Chemokine‑1 Monoclonal Antibody (Catalog # MAB1116). The ND50 is typically 4‑20 µg/mL. |
