| 货号 | MAB5971-SP |
| 别名 | 6030405N23Rik; BGIF; Ifnz; IFN-z; interferon zeta; Lmtn | 全称 | Interferon zeta |
| 反应种属 | Mouse |
| 应用 | ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects mouse Limitin/IFN‑ zeta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma is observed. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize Limitin inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.02-0.1 µg/mL in the presence of 30 pg/mL Recombinant Mouse Limitin. |
| 来源 | Monoclonal Rat IgG2A Clone # 183707 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 319146 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta Leu22-Arg182 Accession # Q9R1T0 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Limitin is a secreted interferon (IFN)-like glycoprotein. It has antiviral activity and selectively inhibits B-lineage lymphoid-cell development both in vivoandin vitro, while having little effect on myelopoiesis or erythropoiesis. Limitin binds to the IFN-alpha/beta receptors and induces IFN regulatory factor-1. Mouse limitin displays 32%, 26% and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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