Human TNF-alpha MAb (Clone 1825) (25 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human TNF-alpha MAb (Clone 1825) (25 UG)

货号： MAB210-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB210-SP
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Human
应用	Neutralization
目标/特异性	Detects human TNF-alpha in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant rhesus macaque TNF-alpha is observed and no cross-reactivity with recombinant cotton rat, mouse, rat, or porcine TNF-alpha is observed. It shows no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA‑A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhRANK Ligand, rhTNF-beta, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI.
使用方法	Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND₅₀) is typically 0.01-0.06 µg/mL in the presence of 0.75 ng/mL Recombinant Human TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Monoclonal Mouse IgG₁ Clone # 1825
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P PLoS Biol, 2012;10(9):e1001395. Species: Human Sample Type: Whole Cells Application: Neut Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. Authors: Nicolaidou V, Wong MM, Redpath AN PLoS ONE, 2012;7(7):e39871. Species: Human Sample Type: Whole Cells Application: Neut KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis. Authors: Navenot JM, Fujii N, Peiper SC Mol. Pharmacol., 2009;75(5):1074-83. Species: Human Sample Type: Whole Cells Application: Neut Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. Authors: Arnold R, Neumann M, Konig W Immunology, 2007;121(1):71-81. Species: Human Sample Type: Whole Cells Application: Neut Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH Dig. Dis. Sci., 2007;52(10):2775-83. Species: Human Sample Type: Whole Cells Application: Neut Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Authors: Ebert EC, Mehta V Cell. Immunol., 2006;241(1):7-13. Species: Human Sample Type: Whole Cells Application: Neut Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia. Authors: Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA Exp. Hematol., 2006;34(10):1312-22. Species: Human Sample Type: Whole Cells Application: Neut Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production. Authors: Tay SS, McCormack A, Rose ML Transplantation, 2004;78(7):987-94. Species: Human Sample Type: Whole Cells Application: Neut A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G J. Leukoc. Biol., 2001;70(4):617-23. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human TNF-alpha Gly57-Leu233 Accession # P01375
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.
运输条件	Blue Ice
存放说明	4℃
参考文献	Adipocytokines Cancer Biomarkers Conventional/Classical Dendritic Cells Co-stimulatory and Co-inhibitory Molecules Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines in Lipid/Cholesterol Metabolism Diabetes Diabetic Peripheral Neuropathy EMT Induction gamma delta T Cells ICC / IHC Images: TNF-alpha Inflammatory Mediators Inflammatory/Monocyte-derived Dendritic Cells Interactive Pathway: Adipocytokines & Insulin Signaling Isolation, Culture, and Identification of Natural Killer Cells Macrophage Activation Markers Natural Killer T (NKT) Cells Neuroinflammation Obesity Plasmacytoid Dendritic Cells Preeclampsia Regulation of Apoptosis by TNF Superfamily Members Regulation of Natural Killer Cell Co-stimulation by TNF Superfamily Members Regulation of T Cell Co-stimulation by TNF Superfamily Members T Cell Cytokine Signaling Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest Th1 Cells Th22 Cells TNF Superfamily Ligands

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF‑ alpha Antibody.
Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF‑ alpha Monoclonal Antibody (Catalog # MAB210). The ND₅₀ is typically 0.01‑0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).