| 货号 | MAB10262-SP |
| 别名 | AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma receptor 1; IFN-gamma-R1; IFNGR; IFNGR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2 | 全称 | Interferon gamma Receptor 1 |
| 反应种属 | Mouse |
| 应用 | ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects mouse IFN-gamma R1/CD119 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) IFN‑ alpha / beta R1, rmIFN-gamma R2, recombinant human (rh) IFN-gamma R1, rhIL-20 R alpha, rhIL-20 R beta, rmIL-20 R alpha, rhIL-22BP or rmIL-22BP is observed. |
| 使用方法 | ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. N. et al. (1982) Infect. Immun. 38:681. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma. |
| 来源 | Monoclonal Rat IgG2B Clone # 170911 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3459 (Human); 15979 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CD40L-expressing CD8 T cells prime CD8alpha(+) DC for IL-12p70 production. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IFN-gamma R1/CD119 Gly23-Asp253 (predicted) Accession # NP_034641 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R1/CD119 Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IFN‑ gamma R1/CD119 Monoclonal Antibody (Catalog # MAB10262). The ND50 is typically 4-20 ng/mL. |
