| 货号 | MAB8762-SP |
| 别名 | IBP6; IBP-6; IGF binding protein 6; IGF-binding protein 6; IGFBP-6; insulin-like growth factor binding protein 6; insulin-like growth factor-binding protein 6 | 全称 | Insulin-like Growth Factor Binding Protein 6 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human IGFBP-6 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) IGFBP-6 is observed, less than 4% cross-reactivity with rmIGFBP-3 and recombinant human (rh) IGFBP-4 is observed, and no cross-reactivity with rhIGFBP‑1, -2, -3, rmIGFBP-1, or -2 is observed. |
| 使用方法 | Neutralization: Measured by its ability to neutralize IGFBP‑6 inhibition of IGF‑II-dependent proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 5‑25 µg/mL in the presence of 0.8 µg/mL Recombinant Human IGFBP‑6 and 14 ng/mL Recombinant Human IGF‑II. |
| 来源 | Monoclonal Mouse IgG2B Clone # 164428 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3489 (Human); 16012 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human IGFBP-6 Ala25-Gly240 Accession # P24592 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-6 cDNA encodes a 240 amino acid (aa) precursor protein with a putative 24 aa signal peptide that is processed to generate the 216 aa mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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IGFBP‑6 Inhibition of IGF‑II-dependent Cell Proliferation and Neutralization by Human IGFBP‑6 Antibody. Recombinant Human IGFBP‑6 (Catalog # 876-B6) inhibits Recombinant Human IGF‑II (Catalog # 292-G2) induced proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF‑II (14 ng/mL) activity elicited by Recombinant Human IGFBP‑6 (0.8 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IGFBP‑6 Monoclonal Antibody (Catalog # MAB8762). The ND50 is typically 5-25 µg/mL. |
