Human BMP-7 MAb (Clone 164311) (25 UG)【科瑞奥博】

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Human BMP-7 MAb (Clone 164311) (25 UG)

货号： MAB3541-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB3541-SP
别名	BMP-7; bone morphogenetic protein 7; Eptotermin alfa; OP-1; OP-1OP1; Osteogenic protein 1
全称	Bone Morphogenetic Protein 7
反应种属	Human
应用	Western Blot,Immunohistochemistry,Neutralization
目标/特异性	Detects human BMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8 is observed.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Neutralization: Measured by its ability to neutralize BMP‑7-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND₅₀) is typically 1.5-6.0 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑7 and 50 µg/mL L-ascorbic acid.
来源	Monoclonal Mouse IgG_2B Clone # 164311
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	655 (Human); 12162 (Mouse); 85272 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade. Authors: Du Y, Xiao Q, Yip HK Invest. Ophthalmol. Vis. Sci., 2010;51(7):3764-73. Species: Mouse Sample Type: Tissue Homogenates Application: WB A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7. Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY J. Mol. Biol., 2008;381(4):1025-39. Species: Human Sample Type: recombinant BMP-7 Application: ELISA Development Targeting of bone morphogenetic protein growth factor complexes to fibrillin. Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY J. Biol. Chem., 2008;283(20):13874-88. Species: Human Sample Type: recombinant BMP-7 Application: WB Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation. Authors: Hsu MY, Rovinsky SA, Lai CY, Qasem S, Liu X, How J, Engelhardt JF, Murphy GF Lab. Invest., 2008;88(8):842-55. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7. Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT J. Cell. Biochem., 2005;95(1):1-16. Species: Mouse Sample Type: Cell Culture Supernates Application: IP
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7 Ser293-His431 Accession # P18075
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
背景	Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but often remains associated with the mature C-terminus. Based on in vivo and in vitro studies, BMP-7 has the potential to be secreted as a disulfide‑linked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides noncovalently associated with a mature disulfide‑linked homodimer (5, 6). The presence of the propeptides in BMP-7 appears to stabilize the molecule and provide a docking mechanism for extracellular storage on molecules such as fibrillin-1 and -2 (5, 6). The propeptides themselves do not impart latency to the complex. BMP-7 binding to type II receptors rapidly displaces the prodomain:mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP-7 (via prodomain:fibrillin) is inactive, allowing for possible long-term storage of the molecule (6). BMP-7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 may also be processed into a disulfide‑linked heterodimer with either BMP-2 or BMP-4. Such complexes may show increased potency and range of activity compared to BMP-7 homodimers (7‑9). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13‑15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
运输条件	Blue Ice
存放说明	4℃
参考文献	Chen, D. et al. (2004) Growth Factors 22:233. Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265. Ozkaynak, E. et al. (1990) EMBO J. 9:2085. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025. Israel, D.I. et al. (1996) Growth Factors 13:291. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181. Grishina, I.B. et al. (2005) Dev. Biol. 288:334. Zeisberg, M. et al. (2003) Nat. Med. 9:964. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

参考图片

Alkaline Phosphatase Production Induced by BMP‑7 and Neutralization by Human BMP‑7 Antibody. Recombinant Human BMP‑7 (Catalog #
354‑BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541). The ND₅₀ is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL).

BMP‑7 in Human Kidney. BMP‑7 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3541) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.