| 货号 | MAB5931-SP |
| 别名 | B cell maturation antigen; B-cell maturation protein; BCMAtumor necrosis factor receptor superfamily member 17; BCMB-cell maturation factor; CD269 antigen; CD269; TNFRSF17; tumor necrosis factor receptor superfamily, member 17 | 全称 | B Cell Maturation Factor |
| 反应种属 | Mouse |
| 应用 | Western Blot,Blockade of Receptor-ligand Interaction |
| 目标/特异性 | Detects mouse BCMA in ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.4 - 1.6 μg/mL of this antibody will block 50% of the binding of 750 ng/mL of recombinant human APRIL to immobilized recombinant mouse BCMA/Fc Chimera coated at 1 μg/mL (100 μL/well). At 20 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Monoclonal Rat IgG2A Clone # 161608 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 608 (Human); 21935 (Mouse); 287034 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse BCMA Met1-Thr49 Accession # O88472 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | BCMA, B cell maturation, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Mouse BCMA is a 185 amino acid (aa) protein consisting of a 49 aa extracellular domain, a 23 aa transmembrane domain, and a 113 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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