货号 | MAB1091-SP |
别名 | CXCL7/Thymus Chemokine-1; Thymus Chemokine1 |
反应种属 | Mouse |
应用 | Western Blot,Neutralization |
目标/特异性 | Detects mouse CXCL7/Thymus Chemokine‑1 in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human (rh) CXCL1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 16, recombinant mouse (rm) CXCL1, 4, 6, 9, 10, 11, 12, 13, 15, 16, recombinant rat (rr) CXCL1, 2, 3, 5, recombinant porcine CXCL8, or recombinant cotton rat CXCL10 is observed. |
使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CXCL7/Thymus Chemokine‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 2-8 µg/mL in the presence of 40 ng/mL Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109. |
来源 | Monoclonal Rat IgG2B Clone # 159742 |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 57349 (Mouse); 246358 (Rat) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | E. coli-derived recombinant mouse CXCL7/Thymus Chemokine‑1 Lys40-Tyr113 Accession # Q9EQI5 |
内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
背景 | CXCL7 is a member of the alpha (CXC) subfamily of chemokines. Mouse CXCL7 cDNA encodes a 113 amino acid precursor protein that shares 72% amino acid sequence identity with rat CXCL7. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Mouse CXCL7/Thymus Chemokine‑1 Antibody. Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (Catalog # 1091-CK) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL7/Thymus Chemokine‑1 aa 48‑109 (40 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL7/Thymus Chemokine‑1 Monoclonal Antibody (Catalog # MAB1091). The ND50 is typically 2-8 µg/mL. |