| 货号 | MAB659-SP |
| 别名 | INHBB; Inhibin, Beta B; Inhibin, Beta-2 |
| 反应种属 | Human |
| 应用 | Western Blot,Neutralization |
| 目标/特异性 | Detects human Activin B beta B subunit in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Inhibin B or rhActivin A is observed. In Western blots, approximately 20% cross-reactivity with rhInhibin B is observed and no cross‑reactivity with rhActivin A or recombinant mouse Activin A is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize Activin B-induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. Schwall, R. H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.6-3.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Activin B beta B subunit. |
| 来源 | Monoclonal Mouse IgG1 Clone # 146807 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3625 (Human); 16324 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Activin B Gly293-Ala407 Accession # Q53T31 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Activins and inhibins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins that were originally purified from gonadal fluids as proteins that stimulated or inhibited, respectively, pituitary follicle stimulating hormone (FSH) release. These proteins have since been shown to have a wide range of biological activities including: mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and reproductive physiology. Activins/inhibins are produced as precursor proteins with an amino-terminal propeptide that is cleaved to release the carboxy-terminal bioactive ligands. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and XenopusbetaD) have been cloned. The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A ( beta A‑ beta A), activin B ( beta B‑ beta B), activin AB ( beta A‑ beta B), inhibin A ( alpha ‑ beta A) and inhibin B ( alpha ‑ beta B). At present, little is known about the contribution of the other beta subunits to activin or inhibin formation and biology. At the amino acid sequence level, the mature human beta B subunit is greater than 98% identical to mouse beta B, while the human and mouse alpha subunits share approximately 80% identity. Similarly to other TGF-beta family members, activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated as type I and type II. Two forms of activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling. Besides activins, Act RII has been shown to bind certain other TGF-beta superfamily members. Inhibin A has been shown to bind with low-affinity to Act RII. The existence of a distinct inhibin-specific receptor and/or signal transduction pathway has been hypothesized. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Hemoglobin Expression Induced by Activin B and Neutralization by Human Activin B Antibody. Recombinant Human Activin B beta B subunit (Catalog # 659-AB) increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Human Activin B beta B subunit (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Activin B beta B subunit Monoclonal Antibody (Catalog # MAB659). The ND50 is typically 0.6-3.0 µg/mL. |
