Human DEP-1/CD148 MAb (Clone 143-41) (25 UG)【科瑞奥博】

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Human DEP-1/CD148 MAb (Clone 143-41) (25 UG)

货号： MAB1934-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB1934-SP
别名	CD148 antigen; CD148; Density-enhanced phosphatase 1; DEP-1; DEP1EC 3.1.3.48; HPTP eta; HPTPeta; HPTP-eta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein tyrosine phosphatase, receptor type, J; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
全称	Density Enhanced Receptor Protein Tyrosine Phosphatase
反应种属	Human
应用	Western Blot,Flow Cytometry,Immunoprecipitation,CyTOF-ready
目标/特异性	Detects human DEP-1/CD148 in Western blots.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Immunoprecipitation: 2.5 µg/500 µg cell lysate
来源	Reconstitute at 0.5 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	5795 (Human); 19271 (Mouse); 29645 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis. Authors: Spring K, Fournier P, Lapointe L, Chabot C, Roussy J, Pommey S, Stagg J, Royal I Oncogene, 2015;34(44):5536-47. Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Immunol. Lett., 2011;134(2):167-73. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.
运输条件	Blue Ice
存放说明	4℃
参考文献	Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680. Sorby, M. et al. (2001) Oncogene 20:5219. Jandt, E. et al. (2003) Oncogene 22:4175. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393. Borges, L.G. et al. (1996) Circ. Res. 79:570. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

参考图片

Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitts lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP‑1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP‑1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.