Human IL-22 MAb (Clone 142928) (25 UG)【科瑞奥博】

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Human IL-22 MAb (Clone 142928) (25 UG)

货号： MAB7821-SP 基本售价： 1467.3 元规格： -

产品信息

概述
货号	MAB7821-SP
别名	Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
全称	Interleukin 22
反应种属	Human
应用	Neutralization
目标/特异性	Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
使用方法	Neutralization: Measured by its ability to neutralize IL‑22-induced IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Nagalakshmi, M.L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 1 ng/mL Recombinant Human IL‑22.
来源	Monoclonal Mouse IgG₁ Clone # 142928
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	50616 (Human); 50929 (Mouse); 500836 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TH17 cytokines induce human airway smooth muscle cell migration. Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway. Authors: Zhang F, Shang D, Zhang Y, Tian Y PLoS ONE, 2011;6(5):e20382. Species: Human Sample Type: Whole Cells Application: Neut Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis. Authors: Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB PLoS ONE, 2010;5(11):e14108. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A J. Immunol., 2010;184(9):4880-8. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	E. coli-derived recombinant human IL-22 Ala34-Ile179 Accession # Q9GZX6
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.5 mg/mL in sterile PBS.
背景	Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
运输条件	Blue Ice
存放说明	4℃
参考文献	Dumoutier, L. et al. (2000) J. Immunol. 164:1814. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

参考图片

IL‑10 Secretion Induced by IL‑22 and Neutralization by Human IL-22 Antibody.

Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL‑10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL‑10 DuoSet ELISA Development Kit (Catalog # DY217B). IL‑10 secretion elicited by Recombinant Human IL‑22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑22 Monoclonal Antibody (Catalog # MAB7821). The ND₅₀ is typically 1-5 µg/mL.